گروه‌بندی و بررسی تمایز بین برنج های هوازی و ایرانی از نظر تحمل به تنش اسمزی در مرحله جوانه زنی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد گروه زراعت و اصلاح نباتات دانشکده علوم کشاورزی دانشگاه گیلان

2 استادیار گروه زراعت و اصلاح نباتات دانشکده علوم کشاورزی دانشگاه گیلان

3 دانشیار گروه تولیدات گیاهی علوم کشاورزی و منابع طبیعی دانشگاه گنبد کاووس

چکیده

جوانه­زنی گیاهان از جمله مراحل مهم در طول دوره رشدی آنهاست که اغلب تحت تأثیر تنش­های محیطی قرار می­گیرد. هدف از پژوهش حاضر شناسایی مؤلفه­های جوانه­زنی بود که بیشترین نقش را در تمایز بین ژنوتیپ­های برنج­های هوازی و ایرانی از نظر تحمل به تنش اسمزی داشتند. آزمون استاندارد جوانه­زنی بذر در سه سطح تنش اسمزی (آب مقطر به عنوان محیط نرمال، 8- و 16-  بار حاصل از مانیتول) و با استفاده از 53 ژنوتیپ برنج (شامل 31 ژنوتیپ از برنج­های هوازی و خارجی و 22 رقم از برنج­های ایرانی) انجام شد. گروه‌بندی ژنوتیپ­ها با استفاده از تجزیه خوشه­ای بر اساس متغیرهای جوانه­زنی نشان داد که در شرایط نرمال و تنش 8- بار ژنوتیپ­ها به سه گروه و در شرایط 16- بار به دو گروه قابل تفکیک هستند. تعدادی از ژنوتیپ­ها نیز توانستند در هر سه شرایط در گروه با متوسط ارزش­های بالاتر از میانگین کل قرار گیرند این ژنوتیپ­ها شامل AE6،AE8 ،AE10 ، AE12، AE13، AE15، AE16،AE18 ،AE29  و  AE30بودند که همه جزء ژنوتیپ­های هوازی و خارجی بودند. سپس با در نظر گرفتن دو گروه شامل ژنوتیپ­های هوازی به همراه ارقام خارجی و ایرانی، تجزیه تابع تشخیص انجام شد. آماره لامبدای ویلک برای شرایط نرمال، 8- و 16-  بار به ترتیب 286/0، 213/0 و 397/0 بود و نشان دهنده وجود تفاوت معنی­دار بین دو گروه از لحاظ خصوصیات جوانه­زنی در هر سه شرایط بود. مطابق نتایج، متغیرهای شاخص بنیه بذر، درصد جوانه­زنی، حداکثر درصد مقدار جوانه­زنی، زمان لازم از کاشت تا رسیدن به 90 و 95 درصد حداکثر جوانه­زنی و انرژی جوانه­زنی بیشترین نقش و ضریب آلومتریک کمترین نقش را در تمایز بین دو گروه داشتند.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of discriminant between aerobic and Iranian rice based on tolerance to drought stress at germination stage using discrimination function analysis

نویسندگان [English]

  • Tayebeh Raiesi 1
  • Atefeh Sabouri 2
  • Hossein Sabouri 3
1 Former M.Sc. Student, Dept. of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Iran
2 Assist. Prof., Dept. of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Iran
3 Assoc. Prof., Dept of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Iran
چکیده [English]

Seed germination is an important step in the growth period that often is influenced by environmental stresses. The objective of this study was to identify the germination components with greatest role in discriminant between Iranian and aerobic rice genotypes based on tolerance to drought stress. The standard test for seed germination was conducted in three levels of osmotic stress (distilled water, -8 and -16 bar using manitol) for 53 rice genotypes (including 31 foreign and aerobic genotypes and 22 Iranian varieties). The various germination characteristics were measured. The grouping of genotypes based on germination components, the genotypes were assigned in the three groups at normal and stress conditions -8 bar and two groups at -16 bar respectively. The results showed that some of genotypes were in the group with average values higher than the general average under all the three conditions. These genotypes includes AE6, AE8, AE10, AE12, AE13, AE15, AE15, AE16, AE18, AE29 and AE30, that all were aerobic and foreign genotypes. Then, genotypes were classified as ‘Aerobic and foreign’ and ‘Iranian’ rice and discriminant functionanalysis was performed. Wilk’s Lambda statistic was estimated 0.286, 0.213 and 0.397 at normal condition, -8 and -16 bar, respectively. Showing the significant difference between the two groups based on germination characteristics. According to the results, the variables of seed vigor, germination percentage, the maximum percentage of germination, planting time required to reaching 90% and 95% of maximum germination and germination energy with greatest role and Allometric coefficient with lowest role were effective to distinguish between the two groups.

کلیدواژه‌ها [English]

  • Aerobic genotypes
  • Cluster analysis
  • Germination components
  • distinguishing groups
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