University of Guilan Cereal Research 2252-0163 6 3 2017 04 21 Identification of molecular markers linked to the genes controlling width and length flag and scond leaves and grain yield in bread wheat under rainfed and supplementary irrigation conditions Identification of molecular markers linked to the genes controlling width and length flag and scond leaves and grain yield in bread wheat under rainfed and supplementary irrigation conditions 271 282 2199 FA Yousef Mohammadi Seyyed Abolghasem Mohammadi Prof., Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Mohammad Moghaddam Prof., Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Mozaffar Rostaei Research Assoc. Prof., Dept. of Cereals, Dryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization, Maragheh, Iran Journal Article 2015 03 28 Drought is the most important abiotic stress affecting growth and production of wheat worldwide. To identify genes controlling grain yield, length and width of flag and second leaves in bread wheat, 121 recombinant inbred lines derived from a cross between drought tolerant Azar2 and high yielding 87Zhong 291 varieties were evaluated under rainfed and supplementary irrigation. Transgressive segregation was observed for all of the studied traits as revealed by phenotypic distribution of the traits. Genetic map consisted of 37 SSR, 16 and 32 AFLP markers covered 1157 cM of wheat genome with an average distance of 17.26 cM between two adjacent markers. Based on composite interval mapping, 59 QTLs were identified for the studied traits under rainfed and supplementary irrigation. For flag leaf length, 4 and 1 QTLs were identified under rainfed and supplementary irrigation, respectively. In addition, 7, 8 and 9 QTLS were mapped for width of flag leaf, length and width of second leaf under rainfed condition, respectively. For grain yield, 20 and 10 QTLs were mapped in the supplementary irrigation and rainfed conditions, respectively. In this study, 8 QTLs were common for the traits under study which could be due to genetic linkage or plieotropy. Considering the importance of genomic region between ISSR25_2-CFA2257 markers in controlling grain yield, with saturation of this region with more markers, their could be used in marker assisted selection program. Drought is the most important abiotic stress affecting growth and production of wheat worldwide. To identify genes controlling grain yield, length and width of flag and second leaves in bread wheat, 121 recombinant inbred lines derived from a cross between drought tolerant Azar2 and high yielding 87Zhong 291 varieties were evaluated under rainfed and supplementary irrigation. Transgressive segregation was observed for all of the studied traits as revealed by phenotypic distribution of the traits. Genetic map consisted of 37 SSR, 16 and 32 AFLP markers covered 1157 cM of wheat genome with an average distance of 17.26 cM between two adjacent markers. Based on composite interval mapping, 59 QTLs were identified for the studied traits under rainfed and supplementary irrigation. For flag leaf length, 4 and 1 QTLs were identified under rainfed and supplementary irrigation, respectively. In addition, 7, 8 and 9 QTLS were mapped for width of flag leaf, length and width of second leaf under rainfed condition, respectively. For grain yield, 20 and 10 QTLs were mapped in the supplementary irrigation and rainfed conditions, respectively. In this study, 8 QTLs were common for the traits under study which could be due to genetic linkage or plieotropy. Considering the importance of genomic region between ISSR25_2-CFA2257 markers in controlling grain yield, with saturation of this region with more markers, their could be used in marker assisted selection program. https://cr.guilan.ac.ir/article_2199_65c3127d02516d6983ac4f288161d735.pdf