University of Guilan Cereal Research 2252-0163 6 4 2016 12 21 Investigatingthe genetic diversity in some of cultivated and wild barely genotypes using AFLP molecular markers Investigatingthe genetic diversity in some of cultivated and wild barely genotypes using AFLP molecular markers 533 544 2284 FA Masomeh Hosseini Mahlagha Ghorbanli Hossein Sabouri AhmadReza Dadras Ali Sattarian Hossein Ali Fallahi Journal Article 2014 06 06 In this study, AFLP markers were used to investigate the genetic diversity among 24 genotypes of barley. The eight primer combinations of EcoRI/Tru1I, totally produced 332 scorable bands that 292 (89.15%) were polymorph. Genetic diversity was estimated between 0.29-0.38 using Nei's gene diversity coefficient. Also, genetic similarity of the genotypes varied from 0.20.0 to 0.56. The highest (0.47%) and lowest (0.26%) polymorphism were obtained using primer combinations of EcoRI+ACG-Tru1I+CGA and EcoRI+ACT- Tru1I+CCG, respectively. Cluster analysis using Jaccard coefficient and UPGMA method assigned genotypes to four main groups. In the first and second groups there were six-rows genotypes and in the third and fourth groups a combination of six- and two-rows genotypes. On the based on similarity matrix the genetic distance between Nimrouz and genotype population Khorasan-Razavi also genotype population of Karaje TN2173 and Line EB-86-3 was low, while the genetic distance between genotype population of Tilabad and cross (F1) ALISOS/CI03909-2 was high. Considering to achieving high rates of polymorphism in the present study can use AFLP marker specially primer combination such as ACG- Tru1I+CGA +EcoRI which produced high level of polymorphism as a powerful tool to distinguish of close genotypes and other barley breeding programs. In this study, AFLP markers were used to investigate the genetic diversity among 24 genotypes of barley. The eight primer combinations of EcoRI/Tru1I, totally produced 332 scorable bands that 292 (89.15%) were polymorph. Genetic diversity was estimated between 0.29-0.38 using Nei's gene diversity coefficient. Also, genetic similarity of the genotypes varied from 0.20.0 to 0.56. The highest (0.47%) and lowest (0.26%) polymorphism were obtained using primer combinations of EcoRI+ACG-Tru1I+CGA and EcoRI+ACT- Tru1I+CCG, respectively. Cluster analysis using Jaccard coefficient and UPGMA method assigned genotypes to four main groups. In the first and second groups there were six-rows genotypes and in the third and fourth groups a combination of six- and two-rows genotypes. On the based on similarity matrix the genetic distance between Nimrouz and genotype population Khorasan-Razavi also genotype population of Karaje TN2173 and Line EB-86-3 was low, while the genetic distance between genotype population of Tilabad and cross (F1) ALISOS/CI03909-2 was high. Considering to achieving high rates of polymorphism in the present study can use AFLP marker specially primer combination such as ACG- Tru1I+CGA +EcoRI which produced high level of polymorphism as a powerful tool to distinguish of close genotypes and other barley breeding programs.

https://cr.guilan.ac.ir/article_2284_e4b1e55b38a849a77141e684cbe3260c.pdf