Mapping of QTLs controlling cold tolerance in rice (Oryza sativa L.)

Kebriyaee, Dina; Aalami, Ali; Samizadeh, Habibollah; Allahgholipour, Mehrzad

Mapping of QTLs controlling cold tolerance in rice (Oryza sativa L.)

Document Type : Research Paper

Authors

¹ Former Graduate Student of Plant Breeding, Dept. of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan,

² Assist. Prof. Dept. of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan,

³ Assoc. Prof., Dept. of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan

⁴ Research Assist. Prof., Rice Research Institute of Iran, Rasht, Iran

Abstract

In this research, 124 individuals of an F₂ population derived from a cross between Domsiah variety (cold susceptible) and PR27137-CR153 line (cold resistant) were evaluated to identify the QTLs controlling cold tolerance in rice. Linkage map were established using 20 polymorphic SSRs and 81 AFLP markers which totally covered 3111 cM of the rice genome with an average distance of 31.67 cM. Composite interval mapping was used to determine the association between markers and traits that overall 13 QTLs were found for cold induced wilting tolerance (CIWT), cold induced necrosis tolerance (CINT), cold induced yellowing tolerance (CIYT) and cold tolerance (CT) traits. Three QTLs on chromosomes 2, 8 and 12 for CIWT, 5 QTLs on chromosomes 1, 3, 3, 10 and 12 for CINT, 3 QTLs on chromosomes 1, 2 and 8 for CIYT and 2 QTLs on chromosomes 3 and 12 for CT were mapped. Two major QTLs, qCIWT12 and qCINT12 on chromosome 12 revealed a significant contribution of phenotypic variance and also markers RM7200 and E64-M59-5 were nearly two QTLs.

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