Investigatingthe genetic diversity in some of cultivated and wild barely genotypes using AFLP molecular markers

Document Type : Research Paper

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Abstract

In this study, AFLP markers were used to investigate the genetic diversity among 24 genotypes of barley. The eight primer combinations of EcoRI/Tru1I, totally produced 332 scorable bands that 292 (89.15%) were polymorph. Genetic diversity was estimated between 0.29-0.38 using Nei's gene diversity coefficient. Also, genetic similarity of the genotypes varied from 0.20.0 to 0.56. The highest (0.47%) and lowest (0.26%) polymorphism were obtained using primer combinations of EcoRI+ACG-Tru1I+CGA and EcoRI+ACT- Tru1I+CCG, respectively. Cluster analysis using Jaccard coefficient and UPGMA method assigned genotypes to four main groups. In the first and second groups there were six-rows genotypes and in the third and fourth groups a combination of six- and two-rows genotypes. On the based on similarity matrix the genetic distance between Nimrouz and genotype population Khorasan-Razavi also genotype population of Karaje TN2173 and Line EB-86-3 was low, while the genetic distance between genotype population of Tilabad and cross (F1) ALISOS/CI03909-2 was high. Considering to achieving high rates of polymorphism in the present study can use AFLP marker specially primer combination such as ACG- Tru1I+CGA +EcoRI which produced high level of polymorphism as a powerful tool to distinguish of close genotypes and other barley breeding programs.

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References

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Cereal Research
Volume 6, Issue 4
December 2016
Pages 533-544

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