Studying expression of some genes associated with drought stress in rice (Oryza sativa L.)

Document Type : Research Paper

Authors

1 Assistant Professor. Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran. Email: mohsenzadeh_mohammad@yahoo.com

2 M.Sc, Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran. Email: s.aghaeepour@yahoo.com

3 Prof., Dept. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

4 Researcher, Agricultural Biotechnology Research Institute of Iran-North Branch, Agricultural Research, Education and Extention Organization, Rasht, Iran

Abstract

To investigate the effect of osmotic stress induced by polyethylene glycol 6000 on seed germination components and identifying drought tolerant and susceptible genotypes, 21 rice genotypes were studied at three levels of osmotic stress (0, -7.5 and -15 MPa) with factorial arrangement in a completely randomized design with three replications. The results indicate that there were significant differences among genotypes in different levels of osmotic stress for all studied traits including radicle length, shoot length, radicle to shoot length ratio, radicle fresh weight, radicle dry weight, shoot fresh weight and shoot dry weight. The effect of genotype and genotype osmotic stress interaction were significant in all studied components. Based on measured traits, the genotypes IR28 and Line 416 were selected as susceptible and tolerant genotypes to osmotic stress, respectively. After RNA extraction and cDNA synthesis, the expression pattern of four drought responsive genes (SOD, FNR, APX and NDH) in two genotypes, IR28 and 416, at seedling stage under treatments of 0, 4, 8, 10 and 24 hours of the dehydration stress were studied using real time PCR technique. The results of this study showed that drought responsive genes had higher expression in the tolerant Line 416 than the sensitive genotype IR28 and the expression of all genes except FNR increased with increasing osmotic stress. Also, APX gene didn’t show the significant expression in either genotype, but showed the highest expression at 24 hours after stress in the sensitive genotype IR28.

Keywords


Abarshahr, M., Rabiei, B. and Samizadeh Lahiji, H. 2011. Assessing genetic diversity of rice varieties under drought stress conditions. Notulae Scientia Biologicae 3: 114-123.##Aliakbari, M. and Razi, H. 2013. Isolation of Brassica napus MYC2 gene and analysis of its expression in response to water deficit stress. Molecular Biology Research Communications 2: 63-71.##Alscher, R. G., Erturk, N. and Heath, L. S. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany 53: 1331-1341.##Arora, A., Sairam, R. K. and Srivastava, G. C. 2002. Oxidative stress and antioxidative system in plants. Current Science 1227-1238.##Asada, K. 1999. The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons. Annual Review of Plant Biology 50: 601-639.##Bonifacio, A., Martins, M. O., Ribeiro, C. W., Fontenele, A. V., Carvalho, F. E. L., Margis‐Pinheiro, M. and Silveira , J. A. G. 2011. Role of peroxidases in the compensation of cytosolic ascorbate peroxidase knockdown in rice plants under abiotic stress. Plant, Cell and Environment 34: 1705-1722.##Boo, Y. C. and Jung, J. 1999. Water deficit-induced oxidative stress and antioxidative defenses in rice plants. Journal of Plant Physiology 155: 255-261.##Ciarmiello, Loredana F., Pasqualina Woodrow, Amodio Fuggi, Giovanni Pontecorvo and Petronia Carillo 2011. Plant genes for abiotic stress. In: Shanker, A. and venkateswarlu, B. (Eds.). Abiotic stress in plants: Mechanisms and adaptations. InTechOpen Publications. pp: 283-308.##Covarrubias, A. A. and Reyes, J. L. 2010. Post‐transcriptional gene regulation of salinity and drought responses by plant microRNAs. Plant, Cell and Environment  33: 481-489.##Danyali, S. F., Moghaddam Vahed, S. M., Alavikia, S. S., Samizadeh Lahiji, H. and Norouzi, M. 2019. Effects of drought on expression patterns of genes encoding the antioxidant enzymes associated with chloroplasts in wheat. Biologia Plantarum 63: 575-585.##Fidalgo, F., Santos, A., Santos, I. and Salema, R. 2004. Effects of long‐term salt stress on antioxidant defence systems, leaf water relations and chloroplast ultrastructure of potato plants. Annals of Applied Biology 145: 185-192.##Fu, J. and Huang, B. 2001. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany 45: 105-114.##Fulda, S., Mikkat, S., Stegmann, H. and Horn, R. 2011. Physiology and proteomics of drought stress acclimation in sunflower (Helianthus annuus L.). Plant Biology 13: 632-642.##Gill, S. S. and Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48: 909-930.##Gunes, A., Pilbeam, D. J., Inal, A. and Coban, S. 2008. Influence of silicon on sunflower cultivars under drought stress, I: Growth, antioxidant mechanisms, and lipid peroxidation. Communications in Soil Science and Plant Analysis 39: 1885-1903.##Hefny, M., Abdel-Kader, D. Z., Abdel-Kader, D. Z., Able, A. J., Sutherl, M. W., Guest, D. I., Acar, D., Turkan, L., Ozdemir, F. and Aebi, H. E. 2000. Water-induced oxidative stress and antioxidant defenses in rice plants. InternationalJournalofPlantBreedingandGenetics 1: 59-71.##Higuchi-Takeuchi, M., Ichikawa, T., Kondou, Y., Matsui, K., Hasegawa, Y., Kawashima, M., Sonoike, K., Mori, M., Hirochika, H. and Matsui, M. 2011. Functional analysis of two isoforms of leaf-type ferredoxin-NADP+-oxidoreductase in rice using the heterologous expression system of Arabidopsis. Plant Physiology 157: 96-108.##Horváth, E. M., Peter, S. O., Joët, T., Rumeau, D., Cournac, L., Horváth, G. V., Kavanagh, T. A., Schäfer, C., Peltier, G. and Medgyesy, P. 2000. Targeted inactivation of the plastid ndhB gene in tobacco results in an enhanced sensitivity of photosynthesis to moderate stomatal closure. Plant Physiology 123: 1337-1350.##Jaleel, C. A., Manivannan, P., Wahid, A., Farooq, M., Al-Juburi, H. J., Somasundaram, R. and Panneerselvam, R. 2009. Drought stress in plants: A review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology 11: 100-105.##Khanna-Chopra, R. and Selote, D. S. 2007. Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than-susceptible wheat cultivar under field conditions. Environmental and Experimental Botany 60: 276-283.##Lehtimäki, N., Lintala, M., Allahverdiyeva, Y., Aro, E. M. and Mulo, P. 2010. Drought stress-induced upregulation of components involved in ferredoxin-dependent cyclic electron transfer. Journal of Plant Physiology 167: 1018-1022.##Livak, K. J. and Schmittgen, T. D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25: 402-408.##Marmagne, A., Brabant, P., Thiellement, H. and Alix, K. 2010. Analysis of gene expression in resynthesized Brassica napus allotetraploids: Transcriptional changes do not explain differential protein regulation. New Phytologist 186: 216-227.##Michel, B. E. and Kaufmann, M. R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology 51: 914-916.##Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7: 405-410.##Mittler, R., Vanderauwera, S., Gollery, M. and Van Breusegem, F. 2004. Reactive oxygen gene network of plants. Trends in Plant Science 9: 490-498.##Mohsenzadeh Golfazani, M., Pasandideh Arjmand, M., Kordrostami, M., Samizadeh Lahiji, H., Hassani Kumle, H. and Rezadoost, M. H. 2018. The effect of iron stress on the relative expression level of SOD, MDHR and GPX1 genes in tolerant and sensitive genotypes of rice. Crop Biotechnology 8: 1-13. (In Persian with English Abstract(.##Morita, S., Kaminaka, H., Masumura, T. and Tanaka, K. 1999. Induction of rice cytosolic ascorbate peroxidase mRNA by oxidative stress: The involvement of hydrogen peroxide in oxidative stress signalling. Plant and Cell Physiology 40: 417-422.##Palatnik, J. F., Valle, E. M. and Carrillo, N. 1997. Oxidative stress causes ferredoxin-NADP+ reductase solubilization from the thylakoid membranes in methyl viologen-treated plants. Plant Physiology 115: 1721-1727.##Pasandideh Arjmand, M., Samizadeh Lahiji, H. and Mohsenzadeh Golfazani, M. 2017. The investigation of some photorespiration genes relative expression in response to drought stress in canola (Brassica napus). Crop Biotechnology 7: 31-42. (In Persian with English Abstract).##Rampino, P., Pataleo, S., Gerardi, C., Mita, G. and Perrotta, C. 2006. Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant, Cell and Environment 29: 2152-2143.##Safaei Chaeikar, S., Rabiei, B. and Rahimi, M. 2018. Evaluation of drought tolerance indices in rice genotypes (Oryza sativa L.). Journal of Crop Breeding 10: 7-18. (In Persian with English Abstract(.##Hosseini Salekdeh, Gh, Siopongco, J., Wade, L. J., Ghareyazie, B. and Bennett, J. 2002. Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2: 1131-1145.##Saeedfar, S., Negahban, M. and Soorestani, M. M. 2015. The effect of drought stress on the essential oil content and some of the biochemical characteristics of anise hyssop. European Journal of Molecular Biotechnology 8: 103-114.##Sales, C. R., Ribeiro, R. V., Silveira, J. A., Machado, E. C., Martins, M. O. and Lagoa, A. M. M. 2013. Superoxide dismutase and ascorbate peroxidase improve the recovery of photosynthesis in sugarcane plants subjected to water deficit and low substrate temperature. Plant Physiology and Biochemistry73: 326-336.##Sarowar, S., Kim, E. N., Kim, Y. J., Ok, S. H., Kim, K. D., Hwang, B. K. and Shin, J. S. 2005. Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens. Plant Science 169: 55-63.##Schwanz, P., Häberle, K. H. and Polle, A. 1996. Interactive effects of elevated CO2, ozone and drought stress on the activities of antioxidative enzymes in needles of Norway spruce trees (Picea abies L., Karsten) grown with luxurious N-supply. Journal of Plant Physiology 148: 351-355.##Sgherri, C. L. M., Maffei, M. and Navari-Izzo, F. 2000. Antioxidative enzymes in wheat subjected to increasing water deficit and rewatering. Journal of Plant Physiology 157: 273-279.##Sharma, P. and Dubey, R. S. 2005. Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation 46: 209-221.##Tohidi-Moghadam, R., Shirani-Rad, A. H., Nour-Mohammadi, G., Habibi, D. and Mashhadi-Akbar-Boojar, M. 2009. Effect of super absorbent application on antioxidant enzyme activities in canola (Brassica napus L.) cultivars under water stress conditions. American Journal of Agricultural and Biological Sciences 4: 215-223.##Vahedi, R., Mohsenzadeh Golfazani, M., Pasandideh Arjmand, M. and Samizadeh Lahiji, H. 2019. Investigation of relative expression of some genes related to iron-induced toxicity in two varieties of rice (Oryza sativa). Crop Biotechnology 9: 15-28. (In Persian with English Abstract(.##Venuprasad, R., Lafitte, H. R. and Atlin, G. N. 2007. Response to direct selection for grain yield under drought stress in rice. Crop Science 47: 285-293.##Wang, N., Qian, W., Suppanz, I., Wei, L., Mao, B., Long, Y, Meng, J., Müller, A. E. and Jung, C. 2011. Flowering time variation in oilseed rape (Brassica napus L.) is associated with allelic variation in the FRIGIDA homologue BnaA.FRI.a. Journal of Experimental Botany 62 (15): 5641-5658.##Wang, P., Duan, W., Takabayashi, A., Endo, T., Shikanai, T., Ye, J.-Y. and Mi, H. 2006. Chloroplastic NAD(P)H dehydrogenase in tobacco leaves functions in alleviation of oxidative damage caused by temperature stress. Plant Physiology 141: 465-474.##Wang, W.-B., Kim, Y.-H., Lee, H.-S., Kim, K.-Y., Deng, X.-P. and Kwak, S.-S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry 47: 570-577.##Xu, G., Liu, D., Chen, J., Ye, X., Ma, Y. and Shi, J. 2008. Juice components and antioxidant capacity of citrus varieties cultivated in China. Food chemistry 106: 545-551.##Yang, B., Srivastava, S., Deyholos, M. K. and Kav, N. N. V. 2007. Transcriptional profiling of canola (Brassica napus L.) responses to the fungal pathogen Sclerotinia sclerotiorum. Plant Science 173: 156-171.##Yao, Z. J., Ye, J. Y. and Mi, H. 2001. The role of chloroplast Ndh complex in resisting heat stress in tobacco strain. Proceedings of The 12th International Congress on Photosynthesis. CSIRO Publishing, Collingwood, Australia.##Zeinali, E., Soltani, A. and Galeshi, S. 2002. Response of germination components salinity stress in oilseed rape (Brassica napus L.). Iranian Journal of Agricultural Science 33: 137-145. (In Persian with English Abstract).##Zhang, J. and Kirkham, M. B. 1996. Antioxidant responses to drought in sunflower and sorghum seedlings. New phytologist 132: 361-373.##