Identification of genomic regions associated with iron accumulation at seed of barley doubled haploid population

Gheitaran Poorsahrigh, Shiva; Mohammadi, Seyed Abolghasem; Sadeghzadeh, Behzad

Identification of genomic regions associated with iron accumulation at seed of barley doubled haploid population

Document Type : Research Paper

Authors

¹ Former Graduate Student, Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University

² Prof., Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University.

³ Dry land Agricultural Research Institute, Maragheh

Abstract

Iron is one of the essential micronutrient, which has an important role in nitrogen fixation and activity of some enzymes such as catalase, peroxidase and cytochrome oxidase. To map QTLs for the traits associated with iron accumulation in barley, 148 doubled haploid lines derived from a cross between Clipper an Australian two rowed cultivar and Sahara3771 Algerian six rowed landrace were evaluated under greenhouse condition and single plant grain yield, 1000 kernel weight, single plant iron concentration and content at grain were measured. To saturate linkage map of the population, 26 retrotransposone markers, IRAP and REMAP markers, 246 SSR and EST-SSR, 238 RFLP and one morphological marker were used and covered 1099.09 cM of barley genome with an average distance of 2.37 cM between two adjacent markers. Transgressive segregation was observed for all traits indicating the presence of desirable parental allele combinations in the progenies. In total, 511 polymorphic markers were mapped in 7 linkage groups. For single plant grain yield, six QTLs were mapped with positive additive effects except one. Three QTLs were identified for seed weight which QTL on 2H with positive additive effect explained 70% of the phenotypic variation. For single plant iron concentration and content at grain six and four QTLs were detected, respectively that five and two QTLs had negative additive effects. QTLs had negative additive effects indicating the role of Sahara3771 alleles in increasing iron accumulation in offspring. Two common genomics regions for the QTLs of some traits were identified which could be due to linkage between the QTLs or their pleiotropic effect. Given the strong linkage markers associated with QTL positioning, these markers can be used in marker-assisted breeding programs.

Keywords