Efficiency of EST-SSR markers in determination genetic diversity and relationships of barley landraces

Abdollahi Sisi, Neyer; Mohammadi, Seyed Abolghasem; Alavi-kia, Seyed Siyamak; Sadeghzadeh, Behzad

Efficiency of EST-SSR markers in determination genetic diversity and relationships of barley landraces

Document Type : Research Paper

Authors

¹ Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz

² Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz and Center of Excellence in Cereal Molecular Breeding university of Tabriz, Tabriz

³ Dryland Agricultural Research Institute (DARI), Maragheh, Iran

Abstract

Landraces are valuable genetic resources in crop breeding program. Determining level of genetic variation and relationships between landraces is necessary for optimal conservation and utilization of these valuable resources. In the present study, genetic diversity and relationships of 119 barley landraces from Iran, China, Egypt, Russia, Pakistan and Spain as well as 25 commercial varieties and breeding lines were evaluated using 22 EST-SSR primer pairs. A total of 87 alleles were amplified with an average of 4.14 alleles per locus in 21 polymorphic EST-SSR loci. Polymorphic information content ranged from 0.23 (GBM1212) to 0.84 (SCSSR09398) with an average of 0.52. The average of gene diversity for the studied markers was 0.58 with range of 0.24 to 0.86. Cluster analysis based on EST-SSR data, using Neighbor-Joining algorithm and Kimura 2-parameter evolutionary distance coefficient assigned the genotypes into five groups. In principal coordinate analysis, the first three coordinates explained about 70.09% of total molecular variance. Biplot presentation of the genotypes based on the two first coordinates confirmed the grouping resulted from cluster analysis. Analysis of molecular variance revealed high within group variance in geographical groups of barley which could be utilized in barley breeding programs. The results indicated that EST-SSR markers as functional markers have acceptable efficiency in analysis of genotypes relationships and determining population structure.

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