Efficiency of marker assisted selection in a rice (Oryza sativa L.) F6 population using linked SSR markers to grain quality characteristics

Document Type : Research Paper

Authors

1 Graduated M. Sc., Dept. of Agronomy and PlantBreeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Prof., Dept. of Agronomy and PlantBreeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Research Assist. Prof.,Dept. of Seed Improvement, Rice Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

Abstract

One of the important objectives in improving rice varieties is improving coocking and eating quality, which plays a key role in it’s marketability and economic value. This research was carried out to indirectly select the high quality linesof a population of F6 generation derived from the cross of Hashemi×Nedarice varieties usingmicrosatellite markers tightly linked to cooking and eating quality traits. In this research, 250 F6 lines in an augmented design together with five prevalent rice varieties as the checks including Neda, Hashemi, Domsiah, Kadus and Ali Kazemi, in a randomized complete block design with three replications were cultivated in two regions, Rice Research Institute of Iran(Rasht, Iran), and ChaparsarRice Station(Mazandaran, Iran),in 2015. Genomic DNA amplification was performedbylinked microsatellite markers tothe genomic regions (genes) controlling rice grain quality traits, includingRM217 and RM225 on chromosome 6, RM234 and RM1364 on chromosome 7 andRM331,RM22804 and RM27181 on chromosome 8.The results of this research showed that among the markers used in current study, the highest accuracy and selection efficiency belonged to the RM331 on chromosome 8 which had the smallest distance with the QTLdetected to amylose content, gel consistency and time to peak viscosity. Afterwards, two SSR markers, RM1364 and RM27181 on chromosomes 7 and 8, respectively, were themost efficient markers.Assessing the genomic intervals among the flanking markers also showed that the flanking markers RM234-RM1364 and RM22804-RM331 were the best marker intervals for the screening of the studied population for the qualitative characteristics. 

Keywords

References

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Cereal Research
Volume 8, Issue 3
December 2018
Pages 307-319

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