Effect of water limitation and application of bio-fertilizer and nano-silicon on yield and some biochemical traits of wheat

Document Type : Research Paper

Authors

1 Ph. D. Student, Dept. of Plant Production and Genetic Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardebil, Iran

2 Prof., Dept. of Plant Production and Genetic Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardebil, Iran

3 Prof., Dept. of Plant Production and Genetic Engineering, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Khozestan, Iran

4 Ph. D. Graduate, Dept. of Plant Production and Genetic Engineering, Faculty of Agriculture, University of Urmia, Iran

Abstract

To study the effect of water limitation and application of bio-fertilizer and nanosilicon on yield and some biochemical characteristics of wheat, a factorial experiment was carried out in a randomized complete block design with three replications in the research field of Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran, in 2018-2019. Experimental factors were included irrigation in three levels (full irrigation as control and irrigation cut-off at 50% of booting and heading stages, based on codes 55 and 43 of the BBCH scale, as moderate and severe water limitation, respectively,), foliar application of nano-silicon (water spraying as control, 30 mg/L or 22.5 mg/m2  and 60 mg/L or 45 mg/m2 nano-silicon) and bio-fertilizer (no application as control, application of mycorrhiza (Glomus mosseae), application of flavobacterium + pseudomonas strain 186, combined application of mycorrhiza and flavobacterium + pseudomonas). The results showed that the highest antioxidant enzymes activity and soluble sugars were obtained by combined application of bio-fertilizers and foliar application of 30 mg/L nano-silicon under the condition of irrigation limitation at booting stage. Under full irrigation condition and non-application of bio-fertilizers and nano-silicon, the activity of catalase, peroxidase and polyphenol oxidase enzymes and soluble sugar content decreased 78, 49, 64 and 74 percent, respectively, compared to severe water limitation at booting stage and combined application of bio-fertilizers and 30 mg/L nano-silicon. Maximum proline content was also obtained by foliar application of 60 mg/L nano-silicon (9.50 µg/g FW) and combined application of bio-fertilizers (9.70 µg/g FW) under severe stress conditions (10.97 µg g Fw-1). Hydrogen peroxide (H2O2) content under severe water limitation at booting stage and non-application of bio-fertilizers and nano-silicon was 90% higher than the application of 60 mg/L nano-silicon and combined application of bio-fertilizers under full irrigation condition. The highest grain yield (4593 kg/ha) was obtained from the foliar application of 30 mg/L nano-silicon and combined application of bio-fertilizers under full irrigation conditions. The results of current study showed that the combined application of bio-fertilizers and nano-silicon can increase wheat grain yield under water limitation conditions by improving plant biochemical characteristics.

Keywords

References

Agarie, S., Hanaoka, N., Ueno, O., Miyazaki, A., Kubota, F., Agata, W. and Kaufman, P. B. 1998. Effects of silicon on tolerance to water deficit and heat stress in rice plants (Oryza sativa L.) monitored by electrolyte leakage. Plant Production Science 1 (2): 96-103.##Ahmad, P. and Prasad, M. N. V. 2012. Abiotic stress responses in plants: Metabolism, productivity and sustainability. Springer, New York.##Ahmed, M., Hassan, F. U., Qadir, G., Shaheen, F. A. and Aslam, M. A. 2017. Response of proline accumulation in bread wheat (Triticum aestivum L.) under rainfed conditions. Journal of Agricultural Meteorol 73 (4): 147-155.##Alexieva, V., Sergiev, I., Mapelli, S. and Karanov, E. 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell and Environment 24: 1337-1344.##Abdelilah, M. and Boumezzough, A. 2017. First detection of potosia opaca larva attacks on Phoenix dactylifera and P. canariensis in Morocco: Focus on pests control strategies and soil quality of prospected palm groves. Journal of Entomology and Zoology Studies 4 (5): 984-991.##Anli, M., Baslam, M., Tahiri, A., Raklami, A., Symanczik, S., Boutasknit, A., Ait-El-Mokhtar, M., Ben Laouane, R., Toubali, S., Ait Rahou, Y., Ait Chitt, M., Oufdou, K., Mitsui, T., Hafidi, M. and Meddich, A. 2020. Biofertilizers as strategies to improve photosynthetic apparatus, growth, and drought stress tolerance in the date palm. Frontiers in Plant Science 11: 1-21.##Asghari, B., Khademian, R. and Sedaghati, B. 2020. Plant growth promoting rhizobacteria (PGPR) confer drought resistance and stimulate biosynthesis of secondary metabolites in pennyroyal (Mentha pulegium L.) under water shortage condition. Scientia Horticulturae 263: 109132.##Asrar, A. W. A. and Elhindi, K. M. 2011. Alleviation of drought stress of marigold (Tagetes erecta) plants by using arbuscular mycorrhizal fungi. Saudi Journal of Biological Sciences 18: 93-98.##Bandeoglu, E., Eyidogan, F., Yucel, M. and Oktem, H. A. 2004. Antioxidant responses of shoots and roots of lentil to NaCl-salinity stress. Plant Growth Regulation 42: 69-77.##Bates, I. S., Waldern, R. P. and Teare, I. D. 1973. Rapid determination of free prolin for water stress studies. Journal Plant and Soil 39: 205-207.##Batool, A., Akram, N. A., Cheng, Z. G., Lv, G.C., Ashraf, M., Afzal, M., Xiong, J. L., Wang, J. Y. and Xiong, Y. C. 2019. Physiological and biochemical responses of two spring wheat genotypes to non-hydraulic root-to-shoot signalling of partial and full root-zone drought stress. Plant Physiology and Biochemistry 139: 11-20.##Batool, T., Ali, S. and Seleiman, M. F. 2020. Plant growth promoting rhizobacteria alleviates drought stress in potato in response to suppressive oxidative stress and antioxidant enzymes activities. Scientific Reports 10: 16975.##Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-day binding. Analytical Biochemistry 72: 248-254.##Bukhari, M. A., Ahmad, Z., Ashraf, M. Y., Afzal, M., Nawaz, F., Nafees, M., Jatoi, W. N., Malghani, N. A., Shah, A. N. and Manan, A. 2020. Silicon mitigates drought stress in wheat (Triticum aestivum L.) through improving photosynthetic pigments, biochemical and yield characters. Silicon 2020. https://doi.org/10.1007/s12633-020-00797-4.##Chakraborty, U., Chakraborty, B. N., Chakraborty, A. P. and Dey, P. L. 2013. Water stress amelioration and plant growth promotion in wheat plants by osmotic stress tolerant bacteria. World Journal of Microbiology and Biotechnology 29: 789-803.##Dubios, M., Gilles, K. A., Hamilton, J. K., Roberts, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Annual of chemistry 28: 350-356.##Ghanbari, M., Mokhtassi-Bidgoli, A., Mansour Ghanaei-Pashaki, K. and Talebi-Siah Saran, P. 2021. The study of yield and physiological characteristics of pearl millet (Pennisetum glaucum) in response to bio-fertilizers and different irrigation regimes. Journal of Agricultural Science and Sustainable Production 31 (1): 23-37.##Gholinezhad, E., Darvishzadeh, R., Siavash Moghaddam, S. and Popovic-Djordjevic, J. 2020. Effect of mycorrhizal inoculation in reducing water stress in sesame (Sesamum indicum L.). The assessment of agrobiochemical traits and enzymatic antioxidant activity. Journal of Agricultural Water Management 238: 106234-106245.##Gianinazzi, S., Schuepp, H., Barea, J. M. and Haselwandter, K. 2001. Mycorrhizal technology in agriculture: From genes to bioproducts. Birkhauser, Basel. 296 p. ISBN: 376436858.##Gong, H. and Chen, K. 2012. The regulatory role of silicon on water relations, photosynthetic gas exchange, and carboxylation activities of wheat leaves in field drought conditions. Acta Physiologiae Plantarum 34: 1589-1594.##Gong, H. J., Chen, K. M. and Zhao, Z. G. 2008. Effects of silicon on defense of wheat against oxidative stress under drought at different developmental stages. Biologia Plantarum 52: 592-596.##Gaur, S., Kumar, J., Kumar, D., Chauhan, D. K., Prasad, S. M. and Seivastava, P. K. 2020. Fascinating impact of silicon and silicon transporters in plants: A review. Ecotoxicology and Environmental Safety 202: 110885.##Haddad, R. and Mokhlesian, S. 2016. Effect of silicon on the peroxidase gene expression and morphological traits of barley under drought Stress. Journal of Cell and Tissue 6 (4): 451 460. (In Persian with English Abstract).##Hajiboland, R., Cherghvareh, L. and Dashtebani, F. 2017. Effect of silicon supplementation on wheat plants under salt stress. Journal of Plant Process and Function 5 (18): 1-12.##Irankhah, S., Ganjeali, A., Mashreghi, M. and Lari, Z. 2021. Mixed inoculum of rhizobacteria and arbuscular mycorrhizal fungus enhance diosgenin contain and phosphorus uptake in fenugreek under drought stress. Rhizosphere 18: 100338.##Jin, R., Wang, Y., Liu, R., Gou, J. and Chan, Z. 2016. Physiological and metabolic changes of purslane (Portulaca oleracea L) in response to drought, heat, and combined stresses. Frontiers in Plant Sciences 6: 1123.##Kalteh, M., Alipour, Z. T., Ashraf, S., Aliabadi, M. M. and Nosratabadi, A. F. 2014. Effect of silica nanoparticles on basil (Ocimum basilicum) under salinity stress. Journal of Chemical Health Risks 4 (3): 49-55.##Karo, M. and Mishra, D. 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology 57: 315-319.##Kheirizadeh Arough, Y., Seyed Sharifi, R. and Seyed Sharifi, R. 2016. Bio fertilizers and zinc effects on some physiological parameters of triticale under water-limitation condition. Journal of Plant Interactions 11 (1): 167-177.##Laxa, M., Liebthal, M., Telman, W., Chibani, K. and Dietz, K. J. 2019. The role of the plant antioxidant system in drought tolerance. Antioxidants 8 (4): 1-31.##Luyckx, M., Hausman, J. F., Lutts, S. and Guerriero, G. 2017. Silicon and plants: Current knowledge and technological perspectives. Frontiers in Plant Sciences 8: 411.##Maghsoudi, K., Emam, Y., Ashraf, M. and Arvin, M. J. 2019. Alleviation of field water stress in wheat cultivars by using silicon and salicylic acid applied separately or in combination. Crop and Pasture Science 70: 36-43.##Meloni, D., Oliva, M., Martinez, C. and Cambraia, J. 2003. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany 49 (1): 69-76.##Miri-Hesar, K., Dadkhodae, A., Dorostkar, S. and Heidari, B. 2019. Differential activity of antioxidant enzymes and physiological changes in wheat (Triticum aestivum L.) under drought stress. Notulae Scientia Biologicae 11 (2): 266-276.##Mittler, R., Vanderauwera, S., Gollery, M. and Vanbreusegem, F. 2004. Reactive oxygen gene network of plants. Trends in Plant Science 9: 490-498.##Moller, I. M., Jensen, P. E. and Hansson, A. 2007. Oxdative modifications to cellular components in plants. Annual Review of Plant Biology 58: 459-481.##Mushtaq, A., Khan, Z., Khan, S., Rizwan, S., Jabeen, U., Bashir, F., Ismail, T., Anjum, S. and Masood, A. 2020. Effect of silicon on antioxidant enzymes of wheat (Triticum aestivum L.) grown under salt stress. Silicon 12: 2783-2788.##Nemat-Alla, M. M., Badawi, A. M., Hassan, N. M., El-Bastawisy, Z. M. and Badran, E. G. 2008. Effect of metribuzin, butachlor and chlorimuron-ethyl on amino acid and protein formation in wheat and maize seedlings. Pesticide Biochemistry and Physiology 90: 8-18.##Raklami, A., Bechtaoui, N., Tahiri, A., Anli, M., Meddich, A. and Oufdou, K. 2019. Use of rhizobacteria and mycorrhizae consortium in the open field as a strategy for improving crop nutrition, productivity and soil fertility. Frontiers in Microbiology 10: 1106.##Sattar, A., Sher, A., Ijaz, M., Ul-Allah, S., Rizwan, M. S. and Hussain M. 2020. Terminal drought and heat stress alter physiological and biochemical attributes in flag leaf of bread wheat. Plos One 15 (5): 1-14.##Selim, D. H., Nassar, R. A., Boghdady, M. S. and Bonfill, M. 2019. Physiological and anatomical studies of two wheat cultivars irrigated with magnetic water under drought stress conditions. Plant Physiology and Biochemistry 135: 480-488.##Shekari, F., Abbasi, A. and Mustafavi, S. H. 2017. Effect of silicon and selenium on enzymatic changes and productivity of dill in saline condition. Journal of the Saudi Society of Agricultural Sciences
16 (4): 367-374.##Siddiqui, M. H., Al-Whaibi, M. H., Firoz, M. and Al-Khaishany, M. Y. 2015. Role of nanoparticles in plants. In: Siddiqui, M. H., Al-Whaibi, M. H. and Firoz, M. (Eds.). Nanotechnology and plant science. Springer. pp: 19-35.##Sonobe, K., Hattori, T., Tsuji, W., Eneji, A. E., Kobayashi, S., Kawamura, Y., Tanaka, K. and Inanaga, S. 2011. Effect of silicon application on sorghum root responses to water stress. Journal of Plant Nutrition 34 (1): 71-82.##Stewart, R. C. and Beweley, J. D. 1980. Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology 65: 245-248.##Wagar, A., Shahroona, B. Z., Zahir, A. and Arshad, M. 2004. Inoculation with Acc deaminase containing rhizobacteria for improvming growth and yield of wheat. Pakistan Journal of Agricultural Sciences 41: 119-124.##Wu, S. C., Caob, Z. H., Lib, Z. G., Cheunga, K. C. and Wong, M. H. 2005. Effects of bio-fertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: A greenhouse trial. Journal of Geoderma 125: 155-166.##Young, L. S., Hameed, A., Peng, S. Y., Shan, Y. H. and Wu, S. P. 2013. Endophytic establishment of the soil isolate Burkholderia sp. CC-Al74 enhances growth and P-utilization rate in maize (Zea mays L.). Applied Soil Ecology 66: 40-47.##
Cereal Research
Volume 10, Issue 4
March 2021
Pages 285-298

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