Effect of covered smut disease on phenological, physiological and agronomic traits of barley cultivars

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran

2 Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran

3 Research Associate Professor, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran

4 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran

5 Research Assistant Professor, Sistan and Blouchestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Zabol, Iran

Abstract

Introduction
Covered smut disease caused by the biotrophic fungus, Ustilago hordei (Pers.) Lagerh., is one of the significant limiting factors in barley cultivation. This disease is present in all barley cultivation regions, and if seeds of sensitive cultivars are planted without the use of fungicides, it can become a serious problem. Moreover, this disease adversely affects grain quality by contamination of healthy seeds with teliospores. The contamination level in the farms of Karaj and Zabol in 1990-1992 ranged from zero to 90%. Considering the importance of organic agriculture and the public demand for production systems with reduced chemicals inputs, planting improved seeds with genetic resistance is the most effective and economical approach to control this disease. This research was conducted to assess the sensitivity of barley cultivars to the covered smut disease and to evaluate the effect of this disease on the phenological, physiological, and agronomic characteristics of barley cultivars.
Materials and methods
 In this study, 148 spring barley varieties from common commercial varieties from European countries along with Oxin and Zehak cultivars from Iran were investigated. This experiment was conducted in field conditions using an alpha lattice design with two replications under two environmental conditions, disinfection of seeds with fungicide and disease stress by inoculation of the covered smut pathogen, in the Zehak Agricultural Research Station, Sistan and Blouchestan province, Iran, in 2021-22 growing season. To induce the stress of covered smut disease, the seeds of the studied cultivars were artificially contaminated. The measured traits in this experiment included phenological, morphological, physiological, and agronomic traits, as well as spike infection percentage after maturity. To perform statistical analyses, the normal distribution of the collected data was firstly tested using the Shapiro-Wilk test, and then combined analysis of variance was conducted. Comparison of means, simple correlation coefficients between traits, principal component analysis, and heritability of the studied traits were conducted.
 
Research findings
The results showed that the accurence rate of covered smut disease among the studied cultivars under disease inoculation conditions varied from 0% to 26.48% with the average of 3.51%. The genotype × environment interaction for all studied traits including days to tillering, tiller number per plant, plant height, flag leaf length and width, flag leaf sheath length, peduncle length, spike length, number of nodes, internode distance, 1000-grain weight, grain yield, biological yield, harvest index, water-soluble carbohydrate concentration, and disease infection percentage, was significant at 1% probability level, except for phenological traits including days to germination, days to tillering, days to heading, as well as canopy temperature. The heritability rate of resistance to covered smut disease was 97.89%. Also, a negative and significant correlation was observed between the percentage of disease infection with plant height, flag leaf sheath length, peduncle length, spike length at the 1% probability level, and with biological yield at the 5% probability level.
Conclusion
The results of this research showed that there was a significant genetic diversity among the evaluated cultivars and the studied cultivars exhibited diverse reactions to different environmental conditions. Due to the significant impact of covered smut disease or loose smut disease on spike-related traits during the maturity stage, therefore it is challenging to differentiate resistant cultivars from susceptible ones in other stages and traits, and the selection of resistant cultivars requires waiting until the end of the cropping season.

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Main Subjects


Ali, S., Laurie, J.D., Linning, R., Cervanteschavez, J.A. and Gaudet, D. 2014. An immunity triggering effector from the barley smut fungus Ustilago hordei resides in an Ustilaginaceae-specific cluster bearing signs of transposable element assisted evolution. PLoS Pathogens, 10(7), e1004223. https://doi.org/10.1371/journal.ppat.1004223.
Dadrezaei, S.T. and Tabatabaei, S.N. 2021. Investigation on resistance to scald and covered smut among the selected genotypes of barley under field conditions in Khuzestan, Iran. Applied Entomology and Phytopathology, 88(2), pp. 241-250. [In Persian]. https://doi.org/10.22092/jaep.2020.341486.1322.
Dumalasova, V., Leona Svobodova, L.L. and Bartos, P. 2014. Common bunt resistance of Czech and European winter wheat cultivars and breeder lines. Czech Journal of Genetics and Plant Breeding, 50(3), pp. 201-207. https://doi.org/10.17221/241/2013-CJGPB.
Emara, Y.A. and Freake, G.W. 1981. Effect of environment and genotype and their interaction on pathogenicity of Ustilago hordei: I. Parasite-environment effects. Journal of Heredity, 72(4), pp. 261-263. https://doi.org/10.1093/oxfordjournals.jhered.a109492.
Fischer, G.W. and Holton, C.S. 1957. Biology and Control of the Smut Fungi. The Ronald Press Company, New York, USA. 622 p.
Ganeva, G., Landjeva, S., Belchev, I. and Koleva, L. 2014. Characterization of two wheat doubled haploid populations for resistance to common bunt and its association with agronomic traits. Cereal Research Communications, 42(3), pp. 484–494. https://doi.org/10.1556/CRC.42.2014.3.11.
Gangwar, O.P., Bhardwaj, S.C., Singh, G.P., Prasad, P. and Kumar, S. 2018. Barley disease and their management: An Indian perspective. Wheat and Barley Research, 10(3), pp. 138-150. https://doi.org/10.25174/2249-4065/2018/83844.
Goates, B J. 1996. Common bunt and dwarf bunt. In: Wil­coxson, R.D. and Saari, E.E. (Eds.). Bunt and Smut Diseases of Wheat: Concepts and Methods of Disease Management. CIMMYT, Mexico. pp. 12-25.
Grewal, T.S., Rossnagel, B.G. and Scoles, G.J. 2006. Inheritance of resistance to covered smut [Ustilago hordei (Pers.) Lagerh.] in barley. Canadian Journal of Plant Science, 86(3), pp. 829-837. https://doi.org/10.4141/P04-196.
Grewal, T.S., Rossnagel, B.G., Bakkeren, G. and Scoles, G.J. 2008. Identification of resistance genes to barley covered smut and mapping of the Ruh1 gene using Ustilago hordei strains with defined avirulence genes. Canadian Journal of Plant Pathology, 30(2), pp. 277-284. https://doi.org/10.1080/07060661.2008.10540543.
Kaur, A., Sharma, V.K., Rani, R. and Mohan, C. 2014. Management of covered smut of barley through cultural, chemical and biological methods. Journal of Mycology and Plant Pathology, 44(4), pp. 432-437.
Mathre, D.E. 1997. Compendium of Barley Diseases. 2nd Edition. American Phytopathological Society. St. Paul, Minnesota, USA. 78 p.
Mourad, A.M.I., Mahdy, E., Bakheit, B.R., Elwafaa, A.A. and Baenziger, P.S. 2018a. Effect of common bunt infection on agronomic traits in wheat (Triticum aestivum L.). Journal of Plant Genetics and Breeding, 2(1), 102.
Mourad, A.M.I., Sallam, A., Belamkar, V., Mahdy, E., Bakheit, B., El-Wafaa, A.A. and Baenziger, P.S. 2018b. Genetic architecture of common bunt resistance in winter wheat using genome wide association study. BMC Plant Biology, 18, 280. https://doi.org/10.1186/s12870-018-1435-x.
Ökmen, B., Mathow, D., Hof, A., Lahrmann, U., Abmann, D.A. and Doehlemann, G. 2018. Mining the effector repertoire of the biotrophic fungal pathogen Ustilago hordei during host and non-host infection. Molecular Plant Pathology, 19(12), pp. 2603-2622. https://doi.org/10.1111/mpp.12732.
Salari, M., Sharifi Tehrani, A., Okhovat, M. and Zad, J. 1993. Evaluation of some fungicides on Ustilago hordei. Proceedings of the 11th Iranian Plant Protection Congress. Aug. 28-Sep. 2, 1993, Rasht, Iran. [In Persian].
Sidhu, G. and Person, C. 1971. Genetic control of virulence in Ustilago hordei. II. Segregation for higher levels of virulence. Canadian Journal of Genetics and Cytology, 13(2), pp.173-178.
Sidhu, G. and Person, C. 1972. Genetic control of virulence in Ustilago hordei. III. Identification of genes for host resistance and demonstration of a gene-for-gene relationship. Canadian Journal of Genetics and Cytology, 14(2), pp. 209-213.
Singh, J., Kaur, A. and Sharma, V.K. 2020. Evaluation of barley genotypes for resistance against covered smut disease. Indian Phytopathology, 73, pp. 359-360. https://doi.org/10.1007/s42360-020-00231-0.
Singh, J., Sharma, V.K., Kaur, A. and Sharma, S. 2021. Differential biochemical response in resistant and susceptible barley (Hordeum vulgare) cultivars upon covered smut (Ustilago hordei) infection. Indian Phytopathology, 74, pp. 173-179. https://doi.org/10.1007/s42360-021-00326-2.
Singh, R.S. 2018. Plant Diseases. Oxford and IBH Press. New Delhi, India. 700 p.
Schlegl, H.G. 1956. Die verwertung orgngischer säuren durch chlorella im licht. Planta, 47, pp. 510-526. https://doi.org/10.1007/BF01935418.
Thomas, P.L. and Menzies, J.G. 1997. Cereal smuts in Manitoba and Saskatchewan, 1989–95. Canadian Journal of Plant Pathology, 19(2), pp. 161-165. https://doi.org/10.1080/07060669709500546.
Wunderle, J., Leclerque, A., Schaffrath, U., Slusarenko, A. and Koch, E. 2012. Assessment of the loose smut fungi (Ustilago nuda and U. tritici) in tissues of barley and wheat by fluorescence microscopy and real-time PCR. European Journal of Plant Pathology, 133, pp. 865-875. https://doi.org/10.1007/s10658-012-0010-9.
Zuo, W., Ökmen, B., Depotter, J.R.L., Ebert, M.K., Redkar, A., Villamil, J.M. and Doehlemann, G. 2019. Molecular Interactions Between Smut Fungi and Their Host Plants. Annual Review of Phytopathology, 57, pp.411-430. https://doi.org/10.1146/annurev-phyto-082718-100139.