Effect of varying nitrogen fertilizer levels on tissue nitrogen content, leaf area, and biomass of rice (Oryza sativa L. cv Hashemi)

Document Type : Research Paper

Authors

1 Department of Plant Production and Genetics Engineering, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Associate Professor, Rice Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

3 DProfessor, Department of Computer Engineering, Faculty of Engineering, University of Guilan, Rasht, Iran

Abstract

Introduction
Optimizing nitrogen fertilizer use is crucial for increasing efficiency and reducing its adverse environmental impacts in rice paddies. However, accurately predicting the timing of nitrogen requirements and effectively managing it in rice cultivation is challenging. Due to low nitrogen use efficiency and the difficulty in precisely forecasting plant needs, only 30-50% of the applied nitrogen is typically absorbed by the crop. Utilizing digital imagery to estimate the nitrogen content in rice leaves can aid farmers in managing fertilization by providing a relatively accurate assessment of the plant's nitrogen status. This approach requires establishing a relationship between the amount of applied nitrogen, the plant's nitrogen content, and the parameters of digital images. Therefore, given the importance of rice cultivation in Guilan and the necessity of optimizing nitrogen use, this study was designed and conducted to investigate the effect of varying nitrogen application rates on nitrogen content, leaf area, and biomass of Hashemi rice in Rasht.
Materials and methods
This experiment was conducted during the 2016-2017 growing season as a randomized complete block design with three replications at the research farm of the Faculty of Agricultural Sciences, University of Guilan, on Hashemi rice. Seven nitrogen levels (0, 30, 45, 60, 75, 90, and 105 kg N/ha from urea) were applied as the experimental treatments. Transplanting occurred on April 30, with three seedlings per hill, spaced 20×20 cm apart, in plots measuring 3×2 meters. Samples were collected at various growth stages to measure plant nitrogen content, leaf area, and dry biomass. The data were analyzed using ANOVA, followed by Duncan's multiple range test for mean comparisons.
Research findings
The analysis of variance revealed that increasing nitrogen application up to 45 kg/ha did not significantly affect plant nitrogen content compared to the control, while 60 kg/ha resulted in a significant difference compared to both 30 kg/ha and the control. Further increases beyond 60 kg/ha had no significant effect on tissue nitrogen content. The findings also indicated that the highest nitrogen content occurred at the tillering and booting stages, while the lowest was observed at the transplanting time. Nitrogen application also had a significant impact on leaf area, with the largest leaf areas recorded at 90 and 105 kg N/ha, and the smallest in the control, which showed no significant difference from the 30 and 45 kg N/ha treatments. The significant interaction effect of nitrogen application and sampling time on biomass indicated that the highest biomass was observed at 105 kg N/ha during the booting stage. Increasing nitrogen not only increased biomass but also altered the proportion of biomass accumulated at different growth stages relative to total biomass.
Conclusion
Overall, the findings of this study suggest that the optimal nitrogen rate for enhancing nitrogen content in rice tissues ranges between 45 and 60 kg N/ha. To achieve at least 95% of the maximum tissue nitrogen content, a minimum of 57.64 kg N/ha is required. This level of nitrogen application not only boosts nitrogen content and plant biomass but also plays a crucial role in improving efficiency and reducing nitrogen losses in rice paddies.

Keywords

Main Subjects


Aber, J. D., Nadelhoffer, K. J., Steudler, P., & Melillo, J. M. (1989). Nitrogen saturation in northern forest ecosystems: excess nitrogen from fossil fuel combustion may stress the biosphere. BioScience, 39(6), 378-386. doi: 10.2307/1311067.##Beech, D., & Norman, M. (1968). The effect of wet season land treatment and nitrogen fertilizer on safflower, linseed, and wheat in the Ord River valley. 2. Safflower and linseed. Australian Journal of Experimental Agriculture, 8(30), 66-71. doi: 10.1071/EA9680066.##Bredemeier, C., & Schmidhalter, U. (2005). Laser-induced chlorophyll fluorescence sensing to determine biomass and nitrogen uptake of winter wheat under controlled environment and field conditions. In: Stafford, J. V. (Ed.). Precision Agriculture. Wageningen Academic Press. pp. 273-280. doi: 10.3920/978-90-8686-549-9_034.##Crews, T. E., & Peoples, M. B. (2005). Can the synchrony of nitrogen supply and crop demand be improved in legume and fertilizer-based agroecosystems? A review. Nutrient Cycling in Agroecosystems, 72(2), 101-120. doi: 10.1007/s10705-004-6480-1.##Evans, H. J., & Burris, R. H. (1992). Highlights in biological nitrogen fixation during the last 50 years. In: Stacey, G. S., Burris, R. H., & Evans H. J. (Eds.). Biological Nitrogen Fixation. Chapman & Hall, New York. pp. 1-42.##Fageria, N. K., & Baligar, V. C. (2001). Lowland rice response to nitrogen fertilization. Communications in Soil Science & Plant Analysis, 32(9-10), 1405-1429. doi: 10.1081/CSS-100104202.##Fairhurst, T., Witt, C., Buresh, R., Dobermann, A., & Fairhurst, T. (2007). Rice: A Practical Guide to Nutrient Management. First Ed. International Rice Research Institute, Manila, Philippines.##FAO. (2022). FAOSTAT Database. Food and Agriculture Organization of the United Nations Production and Protection Series. http://www.faostat.fao.org.##Giller, K. (2001). Cycling of fixed N in tropical cropping systems. In: Giller, K. E. (Ed.). Nitrogen Fixation in Tropical Cropping Systems. Second Edition. CABI Wallingford UK. pp. 93-107. doi: 10.1079/978085199.##Hoffland, E., Dicke, M., Van Tintelen, W., Dijkman, H., & Van Beusichem, M. L. (2000). Nitrogen availability and defense of tomato against two-spotted spider mite. Journal of Chemical Ecology, 26(12), 2697-2711. doi: 10.1023/A:102647.##Jia, L., Chen, X., Zhang, F., Buerkert, A., & Römheld, V. (2004). Use of digital camera to assess nitrogen status of winter wheat in the northern China plain. Journal of Plant Nutrition, 27(3), 441-450. doi: 10.1081/PLN-120028872.##Kaur, N., Dhawan, M., Sharma, I., & Pati, P. K. (2016). Interdependency of reactive oxygen species generating and scavenging system in salt sensitive and salt tolerant cultivars of rice. BMC Plant Biology, 16(1), 131. doi: 10.1186/s12870-016-0824-2.##Kawashima, S., & Nakatani, M. (1998). An algorithm for estimating chlorophyll content in leaves using a video camera. Annals of Botany, 81(1), 49-54. doi: 10.1006/anbo.1997.0544.##Kiss, S., Simihăian, M., Kiss, S., & Simihăian, M. (2002). Compounds tested for evaluation of their inhibiting effect on both soil urease activity and nitrification. In: Kiss, S., & Simihăian, M. (Eds.). Improving Efficiency of Urea Fertilizers by Inhibition of Soil Urease Activity. pp. 221-242. doi: 10.1007/978-94-017-1843-16.##Kumar, S., Shrotria, P., & Deshmukh, J. (2008). Characterizing nutrient management effect on yield of sweet sorghum genotypes. World Journal of Agricultural Sciences, 4(6), 787-789.##Lack, S. H., Naderi, A., Saidat, S. A., Ayenehband, A., Nour-Mohammadi, G. H., & Moosavi, S. H. (2008). The effects of different levels of irrigation, nitrogen and plant population on yield, yield components and dry matter remobilization of corn at climatical conditions of Khuzestan. Journal of Crop Production & Processing, 11(42), 1-14. dor: 20.1001.1.22518517.1386.11.42.1.0. [In Persian].##Lawlor, D. W., Lemaire, G., & Gastal, F. (2001). Nitrogen, plant growth and crop yield. In: Lea, P. J., & Morot-Gaudry, J.-F. (Eds.). Plant Nitrogen. Springer, Berlin, Heidelberg. pp. 343-367. doi: 10.1007/978-3-662-04064-5_13.##Li, Y., Chen, D., Walker, C., & Angus, J. (2010). Estimating the nitrogen status of crops using a digital camera. Field Crops Research, 118(3), 221-227. doi: 10.1016/j.fcr.2010.05.011.##Mahajan, G., Kumar, V., & Chauhan, B. S. (2017). Rice production in India. In: Chauhan, B. S., Jabran, K., & Mahajan, G. (Eds.). Rice Production Worldwide. Springer International Publishing. pp. 53-91. doi: 10.1007/978-3-319-47516-5.##Ministry of Agriculture-Jahad. (2023). Agricultural Statistics. Vol. 1. Crop Plants. Statistics, Information and communication Technology Center, Ministry of Agriculture-Jahad, Tehtan, Iran. [In Persian].##Miranzadeh, H., & Emamm, Y. (2010). Effect of nitrogen and chlormequat chloride on grain yield, phytomass and water use efficiency of four rainfed wheat cultivars. Iranian Journal of Field Crops Research, 8(4), 636-645. doi: 10.22067/gsc.v8i4.795.##Moore Jr, P., Gilmour, J., & Wells, B. (1981). Seasonal patterns of growth and soil nitrogen uptake by rice. Soil Science Society of America Journal, 45(5), 875-879. doi: 10.2136/sssaj1981.03615995004500050010x.##Muñoz-Huerta, R. F., Guevara-Gonzalez, R. G., Contreras-Medina, L. M., Torres-Pacheco, I., Prado-Olivarez, J., & Ocampo-Velazquez, R. V. (2013). A review of methods for sensing the nitrogen status in plants: Advantages, disadvantages and recent advances. Sensors, 13(8), 10823-10843. doi: 10.3390/s130810823.##Prasad, R. (2013). Fertilizer nitrogen, food security, health and the environment. Proceedings of the Indian National Science Academy, 79B(4), 997-1010.##Prasad, R., Kumar, D., Rana, D., Shivay, Y., & Tewatia, R. (2014). Text Book of Plant Nutrient Management. Indian Society of Agronomy, New Delhi. 72 p.##Prasad, R., & Power, J. F. (1995). Nitrification inhibitors for agriculture, health, and the environment. Advances in Agronomy, 54, 233-281. doi: 10.1016/S0065-2113(08)60901-3.##Prasad, R., & Shivay, Y. S. (2015). Fertilizer nitrogen for the life, agriculture and the environment. Indian Journal of Fertilizers, 11(8), 47-53.##Prasad, R., Singh, R., Archna, R., & Singh, D. (2000). Partial factor productivity of nitrogen and its use efficiency in rice and wheat. Fertiliser News, 45(5), 63-65.##Raun, W. R., Solie, J. B., Taylor, R. K., Arnall, D. B., Mack, C. J., & Edmonds, D. E. (2008). Ramp calibration strip technology for determining midseason nitrogen rates in corn and wheat. Agronomy Journal, 100(4), 1088-1093. doi: 10.2134/agronj2007.0288N.##Razavipour, T., Khaledian, M. R., & Rezaei, M. (2018). Effects of nitrogen levels and its splitting on rice yield and nutrient uptake in rice, Hashemi variety. Human & Environment, 16(2), 153-164. [In Persian].##Rezaei, M., Vahed, H. S., Amiri, E., Motamed, M. K., & Azarpour, E. (2009). The effects of irrigation and nitrogen management on yield and water productivity of rice. World Applied Sciences Journal, 7(2), 203-210.##Shaviv, A. (2000). Advances in controlled-release fertilizers. Advances in Agronomy, 7, 1-49, doi: 10.1016/S0065-2113(01)71011-5.##Sims, J. L., & Place, G. A. (1968). Growth and nutrient uptake of rice at different growth stages and nitrogen levels. Agronomy Journal, 60(6), 692-696. doi: 10.2134/agronj1968.00021962006000060033x.##Sinfield, J. V., Fagerman, D., & Colic, O. (2010). Evaluation of sensing technologies for on-the-go detection of macro-nutrients in cultivated soils. Computers & Electronics in Agriculture, 70(1), 1-18. doi: 10.1016/j.compag.2009.09.017.##Soleimani, R. (2008). Effect of rate and time of nitrogen application on grain yield and yield components in spring safflower (Carthamus tinctorious L.). Iranian Journal of Crop Sciences, 10(1), 47-59. dor: 20.1001.1.15625540.1387.10.1.4.7. [In Persian].##Thorp, K. R., Tian, L., Yao, H., & Tang, L. (2004). Narrow-band and derivative-based vegetation indices for hyperspectral data. Transactions of the ASAE, 47(1), 291-299. doi: 10.13031/2013.15854.##Tilman, D., Cassman, K. G., Matson, P. A., Naylor, R., & Polasky, S. (2002). Agricultural sustainability and intensive production practices. Nature, 418(6898), 671-677. doi: 10.1038/nature01014.##Tremblay, N., Fallon, E., & Ziadi, N. (2011). Sensing of crop nitrogen status: Opportunities, tools, limitations, and supporting information requirements. HortTechnology, 21(3), 274-281. doi: 10.21273/horttech.21.3.274.##Ussiri, D., & Lal, R. (2012). Soil Emission of Nitrous Oxide and its Mitigation. Springer, Dordrecht. doi: 10.1007/978-94-007-5364-8.##Ussiri, D., & Lal, R. (2013). Nitrous Oxide Emissions from Rice Fields. In: Soil Emission of Nitrous Oxide and its Mitigation. Springer, Dordrecht. pp. 213-242. doi: 10.1007/978-94-007-5364-8_7.##Zebarth, B., Drury, C., Tremblay, N., & Cambouris, A. (2009). Opportunities for improved fertilizer nitrogen management in production of arable crops in eastern Canada: A review. Canadian Journal of Soil Science, 89(2). doi: 10.4141/CJSS07102.##Zhang, F., Zhang, W., Fan, M., & Wang, J. (2007). Improving fertilizer use efficiency through management practices-Chinese experience. The Fertilizer Association of India Annual Seminar Papers, 5-7 December 2007, New Delhi, India.##Zou, J., Huang, Y., Jiang, J., Zheng, X., & Sass, R. L. (2005). A 3‐year field measurement of methane and nitrous oxide emissions from rice paddies in China: Effects of water regime, crop residue, and fertilizer application. Global Biogeochemical Cycles, 19(2). doi: 10.1029/2004GB002401.##Zubillaga, M., & Urricariet, S. (2005). Assessment of nitrogen status in wheat using aerial photography. Communications in Soil Science & Plant Analysis, 36(13-14), 1787-1798. doi: 10.1081/CSS-200062446.