ویژگیهای بیوشیمیایی و بیان برخی از ژن‌های مسیر اکسیداتیو در برنج (Oryza sativa L) تحت شرایط تنش شوری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران

2 استاد، گروه زراعت و اصلاح نباتات و بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران

3 استادیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران

چکیده

در این مطالعه، بیان ژن‌های گلوتاتیون پراکسیداز (OsGpx) و پراکسی ردوکسین (OsPrx)، میزان H2O2 و میزان آنزیم گایاکول پراکسیداز (POD) در ارقام برنج بومی (حسنی و عنبربو) و اصلاح شده (خزر و نعمت) ایرانی در واکنش به شوری 100 میلی‌مولار ارزیابی و با دو رقم شناخته شده و بین‌المللی متحمل (FL478) و حساس (IR29) به شوری به عنوان شاهدهای آزمایش مقایسه شدند. نتایج نشان داد که میزان فعالیت آنزیم پراکسیداز (POD) در ارقام متحمل در ساعات ابتدایی تنش و در ارقام حساس در ساعات انتهایی تنش به حداکثر خود رسید، به طوری که تحت شرایط تنش شوری فعالیت این آنزیم در ارقام حساس نسبت به ارقام متحمل بیشتر بود. مطالعه بیان ژن گلوتاتین پراکسیداز نشان داد که بیشترین میزان بیان این ژن در ریشه ارقام متحمل در شش ساعت پس از اعمال تنش بود و سپس در زمانهای 12، 24، 48 و 72 ساعت پس از تنش، میزان بیان این ژن به طور قابل ملاحظه‌ای کاهش یافت. در مقابل، بیان این ژن در ارقام حساس با افزایش زمان تنش به تدریج افزایش پیدا کرد. مطالعه ژن پراُکسی ردوکسین نشان داد که میزان بیان این ژن در ارقام متحمل در ساعات اولیه پس از اعمال تنش هیچ تغییری نکرد، اما در ساعت پایانی اعمال تنش (72 ساعت) بیان این ژن به نحو چشم‌گیری افزایش یافت. در مقابل، الگوی ثابتی برای بیان این ژن در ارقام حساس مشاهده نشد. همچنین نتایج نشان داد که در ارقام متحمل، بین کاهش H2O2 و افزایش بیان ژن‌ها و آنزیم‌ گلوتاتیون پراکسیداز رابطه مستقیمی وجود داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Biochemical characteristics and expression of some oxidative pathway genes in rice (Oryza sativa L.) under salt stress condition

نویسندگان [English]

  • Mojtaba Kordrostami 1
  • Babak Rabiei 2
  • Seyyed Hassan Hassani Kumleh 3
1 Ph. D. Candidate, Dept. of Agricultural Biotechnology, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
2 Prof., Dept. of Agronomy and Plant Breeding and Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Assist. Prof., Dept. of Agricultural Biotechnology, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
چکیده [English]

In this research, the expression of glutathione peroxidase (OsGpx) and proxy redoxin (OsPrx), H2O2 and Guaiacol dependent peroxidase (POD) content was measured in Iranian local (Hassani and Anbarboo) and improved (Nemat and Khazar) rice varieties with two well-known international salt tolerant (FL 478) and sensitive (IR29) varieties as checks in response to 100 mM salt stress. The results showed that the activity of peroxidase (POD) was different at different times in the studied cultivars, so that the enzyme activity in resistant cultivars was higher than susceptible ones under salt stress. Gene expression of glutathione peroxidase (OsGpx) showed the highest expression of this gene in the roots of tolerant varieties 6 hours after the stress treatment and then 12, 24, 48 and 72 hours after the stress the expression of this gene significantly decreased. In susceptible cultivars, gene expression increased gradually. The study of peroxy-redoxin (OsPrx) gene expression showed that in the first hours after the stress, tolerant varieties did not show any change in this gene expression, but in the final hours of stress (72 hours) gene expression was significantly increased. There was no consistent pattern for this gene in susceptible cultivars. The results showed there was a direct relationship between the reduction of H2O2 and increased activity of antioxidant genes and enzymes.

کلیدواژه‌ها [English]

  • Glutathione peroxidase
  • Guaiacol peroxidase
  • H2O2
  • Peroxy-redoxin
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