نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
2 دانشیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
3 استاد، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
4 دانش‌آموخته دکتری، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Introduction
Plants are often exposed to a various of biotic and abiotic stresses. Salinity, drought, and temperature stresses, as the most important abiotic factors, have a negative impact on the physiological and biochemical characteristics of plants and reduce crop growth and productivity. Identifying key characteristics related to biotic and abiotic stresses is important in understanding plant responses at the molecular and cellular levels. Studying miRNA components in crop plants is an effective tool for early detection of stresses, physiological changes, and regulation of gene expression. The objective of this study was to identify key genes and pathways involved in drought stress tolerance and introduce them for breeding rice plants for drought stress tolerance.
Materials and Methods
In this study, microarray data of two rice cultivars, drought-tolerant (Dagad deshi) and drought-sensitive (IR20), were analyzed under drought stress. Differentially expressed genes in these two genotypes were identified using the GEO2R tool. Biological pathways, cellular components, and molecular functions related to drought tolerance in the tolerant cultivar were determined, and key genes and their regulating miRNAs were analyzed.
Research findings
Microarray data analysis revealed that under drought stress conditions, 33% of genes were upregulated and 26% downregulated in the tolerant cultivar (Dagad deshi), while in the sensitive cultivar (IR20), 7% of genes were upregulated and 6% were downregulated with significant expression changes. Additionally, 766 genes exhibited upregulation and 340 genes showed downregulation with significant expression changes in both cultivars. The most significant biological processes, cellular components, and molecular functions among the upregulated genes in the tolerant cultivar included response to salicylic acid, cytoplasm, and carboxylase activity, while downregulated genes included monomethylation of peptide lysine, large mitochondrial ribosomal subunit, and long-chain Acyl-CoA reductase activity forming alcohol. KEGG pathway enrichment analysis revealed that the upregulated and downregulated genes in the tolerant cultivar were enriched in 18 and 10 pathways, respectively. Fifteen key, MCM5، Os05g0358200، MCM4، MCM7، NAC037، OS11G0128400، CDC6، RPA2A، OS12G0124700، MCM3، POLA، Os07g0406800، MCM6، Os05g0160800 و PCNA genes were identified as contributors to drought stress resistance pathways, several of which belonged to the MCM gene family. Finally, 247 miRNAs such as osa-miR172c, osa-miR1849, osa-miR2925, osa-miR397a and osa-miR397a were investigated for genes involved in the drought stress resistance pathway.
Conclusion
The key genes and miRNAs identified in this study, which play a role in drought stress tolerance, seem to have the potential to be used for breeding and developing drought-tolerant rice plants.
کلیدواژه‌ها [English]
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