تجزیهQTLبرخی صفات فیزیولوژیک و بیوشیمیایی مرتبط با عملکرد دانه در گندم تحت شرایط تنش گرمای انتهای فصل

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دکتری،گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران (آدرس جدید: گروه کشاورزی، دانشگاه پیام نور، تهران، ایران)

2 دانشیار،گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

3 استاد،گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

4 استادیار،گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

چکیده

به­منظور نقشه­یابی نواحی ژنومی اصلی و اپیستاتیک و برهمکنش آن­ها با محیط برای برخی صفات فیزیولوژیک و بیوشیمیایی گندم، آزمایشی در قالب دو طرح آلفا لاتیس با دو تکرار در دو شرایط نرمال و تنش گرمای انتهای فصل در مزرعه تحقیقاتی مرکز تحقیقات کشاورزی و منابع طبیعی سیستان در سال زراعی 95-1394 اجرا شد. مواد گیاهی آزمایش شامل 167 لاین خویش­آمیخته نوترکیب به­همراه والدین آن­ها ‘‘Babaxو‘SeriM82’بودند که در آن­ها صفات عملکرد دانه، محتوای پرولین برگ،کربوهیدرات­های محلول در آب، حداکثر کارایی فتوسیستمII، پایداری غشاء سیتوپلاسمی ومحتوای کلروفیل برگ اندازه­گیریشد. تجزیه QTLبه روش ترکیبی بر اساس مکان­یابی فاصله­ای مرکب (MCIM) برایهردو محیط به­طور هم­زمان انجام گرفت. اثر اصلی ژنوتیپ برای کلیه صفات مورد مطالعه معنی­دار بود و تفکیک متجاوز از والدین در دو جهت مثبت و منفی مشاهده شد. در تجزیه QTL به­طور هم­زمان برای دو محیط، درمجموع بیست و سه QTL اصلی مکان­یابی شد و برهمکنشQTL×محیط در پنج QTL اصلی معنی­دار شد.همچنین دو جفت QTLدارای اثر اپیستازی برای صفات مورد مطالعه شناسایی شد. اغلبQTL­های شناسایی شده از پایداری لازم برخوردار بودند و بنابراین می­توانند در گزینش به کمک نشانگر برای ایجاد ارقام پرمحصول و متحمل به گرما مورد استفاده قرار گیرند.

کلیدواژه‌ها


عنوان مقاله [English]

QTL analysis of some physiological and biochemical traits associated with grain yield in wheat under terminal heat stress

نویسندگان [English]

  • Faramarz Sohrabi Chah-Hassan 1
  • Mahmood Solouki 2
  • Baratali Fakheri 3
  • Nafiseh Mehdinejad 4
1 Graduated Ph. D., Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran (current adderess: Dept. of Agriculture, Payam-e-Noor University, Tehran, Iran)
2 Assoc. Prof., Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
3 Prof., Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
4 Assist. Prof., Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
چکیده [English]

To identify the genomic regionswith main, epistatic, and QTL×environment (Q×E) interaction effects for some of the physiological and biochemical traits of wheat, an experiment was conductedat Research Farm of Agricultural and Natural Resources Research Center of Sistan, Iran, in 2015-16 growing season. The experiment was carried outusing two alpha lattice designs with two replications under non-stress and terminal heat stress conditions.Plant materials were 167 recombinant inbred lines andtheir parents (‘SeriM82’and ‘Babax’) andsix traits including grain yield (GY), proline content(PRO), water soluble carbohydrates (WSC), maximum efficiency of photosystem II (Fv/Fm), cytoplasmic membrane stability (CMS) and chlorophyll content (CHL) were measured. QTL analysis was conducted by Mixed-Model basedcomposite interval mapping (MCIM) method. There were significant differences among the genotypes for all studied traits and transgressive segregations in both directions were also observed. Using mixed linear model,a total of 23 main-effect QTLs were detected. The Q×E interaction was significant in five main-effect QTLs and two epistatic QTLs were also detectedfor studied traits. The most of mapped QTLsappear to be quite stable. Therefore, can be used in marker-assisted selection (MAS) in order to produce high-performance and heat tolerant wheat genotypes.

کلیدواژه‌ها [English]

  • Epistatic effects
  • QTL×environment interaction
  • Recombinant inbred lines
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