مطالعه بیان ژن‌های ZIP1، ZIP3 و ZIP6 در گندم نان تحت شرایط کمبود روی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشکده کشاورزی

2 دانشگاه ارومیه- دانشکده کشاورزی-گروه اصلاح و بیوتکنولوژی گیاهی

3 گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه ارومیه

4 گروه علوم خاک، دانشکده کشاورزی، دانشگاه ارومیه

چکیده

کمبود روی (Zn) در خاک، عملکرد گیاه و محتوای روی دانه را کاهش می­دهد. تنظیم بیان ژن­های خانواده ZIP (ZRT/IRT-like protein)، یکی از مکانیسم­های عمده گیاهان برای مقابله با تنش کمبود روی است. به­منظور مطالعه بیان ژن­های ZIP1، ZIP3 و ZIP6 در برگ و ریشه ارقام گندم روی-کارا و روی-ناکارا تحت شرایط کمبود روی، آزمایشی به­صورت فاکتوریل بر پایه طرح کاملاً تصادفی با سه تکرار اجرا شد. ارقام بیات و نیک­نژاد (روی-کارا) و هیرمند و کرج 1 (روی-ناکارا) در شرایط کفایت روی و کمبود آن کشت و بیان نسبی این ژن­ها در ریشه و برگ در دو مرحله رشدی، 28 روز بعد از جوانه­زنی (رویشی) و 30 درصد سنبله­دهی (زایشی)، اندازه­گیری شد. نتایج نشان داد که میزان بیان نسبی هر سه ژن تحت شرایط کمبود روی در ارقام روی-کارا به­طور معنی­داری (P≤0.01) بیش­تر از ارقام روی-ناکارا افزایش یافت. بیان نسبی ژن ZIP1 در مراحل رویشی و زایشی در ریشه به­طور معنی­داری (P≤0.01) بیش­تر از برگ بود، در حالی­کهژن­های ZIP3 و ZIP6 همدر ریشه و هم در برگ بیان شدند. تجزیه همبستگی بیان ژن­ها و محتوای Zn دانه نشان داد که همبستگی مثبت و معنی­داری (P≤0.05) بین بیان ژن ZIP6 در برگ در مرحله زایشی و محتوای Zn دانه گندم وجود دارد. به­طور کلی، افزایش بیان ژن ZIP1 در ریشه و ژن­های ZIP3 و ZIP6 در ریشهو برگ در این تحقیق نشان داد که احتمالاً ژن ZIP1 عمدتاً در  جذب روی از خاک و ژن­های ZIP3 وZIP6 درجذب و انتقال روی به اندام­های هوایی در گندم نان دخالت دارند.

کلیدواژه‌ها


عنوان مقاله [English]

Studying the expression of ZIP1, ZIP3 and ZIP6 genes in bread wheat under Zn deficiency conditions

نویسندگان [English]

  • Seyyed Mohsen Niazkhani 1
  • Babak Abdollahi Mandoulakani 2
  • Morad Jafari 3
  • MirHassan Rasouli-Sadaghiani 4
1 Faculty of Agriculture
2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University
3 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University
4 Department of Soil Science, Faculty of Agriculture, Urmia University
چکیده [English]

Zn deficiency in the soil reduces plant yield and grain Zn content. The regulation of ZIP (ZRT/IRT-like protein) family genes is one of the main mechanisms of plant to confront to Zn deficiency stress. To investigate the expression of ZIP1, ZIP3 and ZIP6 genes in roots and leaves of Zn-efficient and inefficient wheat cultivars under Zn deficiency conditions, a factorial experiment based on completely randomized design (CRD) with three replications was conducted in greenhouse. Bayat and Niknejad (Zn-efficient) and Hirmand and Karaj 1 (Zn-inefficient) cultivars were grown under Zn deficiency and adequacy conditions and the relative expression of the genes were measured in root and leaf at two growth stages, 28 days after germination (vegetative) and 30% of flowering (reproductive). The results revealed a significant (P≤0.01) increase in expression of all three studied genes in Zn-efficient than -inefficient cultivars under Zn deficiency conditions. The expression of the ZIP1 in root was significantly more than that of leaf in both vegetative and reproductive stages, while ZIP3 and ZIP6 genes were expressed in both root and leaf. A significant (P≤0.05) and positive correlation was observed between ZIP6 expression in leaves and seed grain Zn content at reproductive stage. In conclusion, with regard to the increased expression of ZIP1 in root and ZIP3 and ZIP6 in both root and leaf, it may be resulted that ZIP1 have a major role in Zn uptake from the roots, while ZIP3 and ZIP6 probably involved in Zn uptake and translocation.


کلیدواژه‌ها [English]

  • Grain Zn content
  • Real Time PCR
  • Zn transporter genes
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