تأثیر تنش خشکی بر بیان دو miRNA مهم در بساک دو ژنوتیپ گندم متفاوت از نظر تحمل به خشکی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

2 دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

3 استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

چکیده

قسمت‌ زیادی از اراضی زیرکشت گندم در جهان تحت تاثیر تنش خشکی قرار دارند. مرحله زایشی، حساس‌ترین مرحله به تنش خشکی در غلات به­شمار می­آید. با وجود اهمیت تنش خشکی در مرحله زایشی و نقش آن در کاهش عملکرد گندم، مکانیسم­های مولکولی درگیر در آن کم­تر مورد مطالعه قرار گرفته است. در مطالعه حاضر، بیان miR166 و miR167 در بساک رسیده دو ژنوتیپ گندم دزفول (D-10) (متحمل به خشکی) و شیراز (حساس به خشکی) تحت شرایط تنش خشکی در مرحله زایشی مورد بررسی قرار گرفت.شاخص تحمل به تنش(STI) برای تعداد دانه در سنبله، مجموع دانه و زنده‌مانی دانه گرده در ژنوتیپ دزفول بالاتر از ژنوتیپ شیراز بود. بیان هر دو miRNA در هر دو ژنوتیپ،تحت تاثیر تنش خشکی افزایش یافت. میزان افزایش بیان miR166 در ژنوتیپ حساس شیراز (افزایش بیش از 14 برابر نسبت به شرایط بدون تنش) حدود دو برابر بیش­تر از ژنوتیپ متحمل دزفول (افزایش حدود 8 برابر نسبت به شرایط بدون تنش) بود. تحت شرایط تنش خشکی،بیان miR167 در ژنوتیپ دزفول بیش از دو برابر و در ژنوتیپ شیراز بیش از چهار برابر نسبت به شرایط بدون تنشافزایش داشت.miR166 و miR167 هر دو در تنظیم نمو اندام‌های گل، مانند نمو میکروسپور، دخالت دارند. بنابراین، اثر تنش خشکی بر تغییر میزان بیان آن‌ها می‌تواند در نرعقیمی حاصله در گندم دخالت داشته باشدو تعدیل بیان این‌دو miRNA می‌تواند منجر به کارایی نمو دانه گرده و شکوفایی موفق بساک شود که به نوبه خود ریشه در ترارسانی دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of drought stress on expression oftwo important miRNAs in anther of two different wheat genotypes in term of drought stress

نویسندگان [English]

  • Nastaran Mehri 1
  • Reza Fotovat 2
  • Ehsan Mohseni Fard 3
1 Ph.D. Student, Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Assoc. Prof., Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Assist. Prof.,Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
چکیده [English]

A large amount of the world lands under the cultivation of wheat is under drought stress. In cereals, the reproductive stage is the most sensitive phase to drought stress. Despite the importance of drought stress in reproductive stage and its role in wheat yield reduction, the mechanisms involved in it have been less studied. In the present study, the expression of miR166 and miR167 in mature anthers of two tolerant (Dezfoul or D-10) and susceptible (Shiraz) wheat genotypes under drought conditions in reproductive stage was investigated. Stress tolerance index (STI) for grain number per spike, grain set and pollen viability was higher in tolerant genotype, Dezfoul, compared to susceptible genotype, Shiraz. Drought stress induced expression level of both miRNAs in both genotypes. The amount of miR166 increase in the susceptible genotype, Shiraz (over 14 times compared to the non-stress conditions) was about twice more than the tolerant genotype,Dezfoul (about 8 timesmore than the non-stress conditions). Under drought stress conditions, expression of miR167 in Dezfoulgenotype was more than two fold and in Shiraz genotype more than four timeshigher thanthe non-stress conditions. Both miR166 and miR167 regulate development of floral organs such as microspore development. In conclusion, the effect of drought stress on their expression changes can affect male sterility in wheat, and modifying the expression of these two miRNAscan lead to efficiency of pollen development and successful anther dehiscence which is rooted in signal transduction.

کلیدواژه‌ها [English]

  • Gene Expression
  • Male sterility
  • Meiosis
  • Pollen viability

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Akdogan, G., Tufekci, E. D., Uranbey, S. and Unver, T. 2016. miRNA-based drought regulation in wheat. Functional andIntegrative Genomics 16: 221-233.##Alexandratos, N. and Bruinsma, J. 2012. World agriculture towards 2030/2050: The 2012 revision, ESA Working Paper,FAO,Rome, Italy.##An, F. M., Hsiao, S. R. and Chan, M. T. 2011. Sequencing-based approaches reveal low ambient temperature-responsive and tissue-specific microRNAs in phalaenopsis orchid. PLoS One 6:e18937.##Ariel, F. D., Manavella, P. A., Dezar, C. A. and Chan, R. L. 2007. The true story of the HD-Zip family. Trends in Plant Science 12: 419-426.##Bakhshi, B., Fard, E. M., Gharechahi, J., Safarzadeh, M., Nikpay, N., Fotovat, R., Azimi, M. R. and Salekdeh, G. H. 2017. The contrasting microRNA content of a drought tolerant and a drought susceptible wheat cultivar. Journal of Plant Physiology 216: 35-43.##Barnabás, B., Jäger, K. and Fehér, A. 2008. The effect of drought and heat stress on reproductive processes in cereals. Plant, Cell andEnvironment 31: 11-38.##Cecchetti, V., Altamura, M. M., Falasca, G., Costantino, P. and Cardarelli, M. 2008. Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation. The Plant Cell 20: 1760-1774.##Chhun, T., Aya, K., Asano, K., Yamamoto, E., Morinaka, Y., Watanabe, M., Kitano, H., Ashikari, M., Matsuoka, M. and Ueguchi-Tanaka, M. 2007. Gibberellin regulates pollen viability and pollen tube growth in rice. The Plant Cell19: 3876-3888.##Ding, Y., Tao, Y. and Zhu, C. 2013. Emerging roles of microRNAs in the mediation of drought stress response in plants. Journal of Experimental Botany 64: 3077-3086.##Dorion, S., Lalonde, S. and Saini, H. S. 1996. Induction of male sterility in wheat by meiotic-stage water deficit is preceded by a decline in invertase activity and changes in carbohydrate metabolism in anthers. Plant Physiology 111: 137-145.##FAO. 2016. Statistics: FAOSTAT agriculture. Food and Agriculture Organization. Retrieved September 20, 2018, from http://fao.org/crop/statistics.##Fard, E. M., Bakhshi, B., Farsi, M., Kakhki, A. M., Nikpay, N., Ebrahimi, M. A., Mardi, M. and Salekdeh, G. H. 2017. MicroRNAs regulate the main events in rice drought stress response by manipulating the water supply to shoots. Molecular Biosystems 13: 2289-2302.##Fotovat, R., Alikhani, M., Valizadeh, M., Mirzaei, M. and Salekdeh, G. H. 2017. A proteomics approach to discover drought tolerance proteins in wheat pollen grain at meiosis stage. Protein and Peptide Letters 24: 26-36.##Fujioka, T., Kaneko, F., Kazama, T., Suwabe, K., Suzuki, G., Makino, A., Mae, T., Endo, M., Kawagishi-Kobayashi, M. and Watanabe, M. 2008. Identification of small RNAs in late developmental stage of rice anthers. Genes andGenetic Systems 83: 281-284.##Grant-Downton, R., Hafidh, S., Twell, D. and Dickinson, H. G. 2009. Small RNA pathways are present and functional in the angiosperm male gametophyte. Molecular Plant 2: 500-512.##Huntzinger, E. and Izaurralde, E. 2011. Gene silencing by microRNAs: Contributions of translational repression and mRNA decay. Nature Reviews Genetics 12: 99-110.##Ji, L., Liu, X., Yan, J., Wang, W., Yumul, R. E., Kim, Y. J., Dinh, T. T., Liu, J., Cui, X. and Zheng, B. 2011. ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis. PLoS Genetics 7: e1001358.##Ji, X., Shiran,B., Wan, J., Lewis, D. C., Jenkins, C. L., Condon, A. G., Richards, R. A. and Dolferus, R. 2010. Importance of preanthesis anther sink strength for maintenance of grain number during reproductive stage water stress in wheat. Plant, Cell and Environment 33: 926-942.##Jian-Chang, Y., Kai, L., Zhang, S. F., Xue-Ming, W., Zhi-Qin, W. and Li-Jun, L. 2008. Hormones in rice spikelets in responses to water stress during meiosis. Acta Agronomica Sinica 34: 111-118.##Jung, J. H. and Park, C. M. 2007. MIR166/165 genes exhibit dynamic expression patterns in regulating shootapical meristem and floral development in Arabidopsis. Planta 225: 1327-1338.##Kim, J., Jung, J. H., Reyes, J. L., Kim, Y. S., Kim, S. Y., Chung, K. S., Kim, J. A., Lee, M., Lee, Y. and Narry Kim, V. 2005. microRNAdirected cleavage of ATHB15 mRNA regulates vascular development in Arabidopsis inflorescence stems. The Plant Journal 42: 84-94.##Koonjul, P., Minhas, J., Nunes, C., Sheoran, I. and Saini, H. 2005. Selective transcriptional down-regulation of anther invertases precedes the failure of pollen development inwater-stressed wheat. Journal of Experimental Botany 56: 179-190.##Krizek, B. A. 2011. Auxin regulation of Arabidopsis flower development involves members of the AINTEGUMENTA-LIKE/PLETHORA (AIL/PLT) family. Journal of Experimental Botany
62: 3311-3319.
##Lee, R. C., Feinbaum, R. L. and Ambros, V. 1993. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75: 843-854.##Li, B., Qin, Y., Duan, H., Yin, W. and Xia, X. 2011. Genome-wide characterization of new anddrought stress responsive microRNAs in Populus euphratica. Journal of Experimental Botany 62: 3765-3779.##Li, Q. J., Xu, B., Chen, X. Y. and Wang, L. J. 2007. The effects of increased expression of an ArabidopsisHD-ZIPgeneonleafmorphogenesisandantherdehiscence.PlantScience173:567-576.##Li, Z. F., Zhang, Y. C. and Chen, Y. Q. 2015. miRNAs and lncRNAs in reproductive development. Plant Science 238: 46-52.##Lian, H., Li, X., Liu, Z. and He, Y. 2013. HYL1 is required for establishment of stamen architecture with four microsporangia in Arabidopsis. Journal of Experimental Botany 64: 3397-3410.##Liu, F., Andersen, M. N. and Jensen, C. R. 2003. 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