اثر سمیت آهن بر برخی صفات مرفولوژیک، بیان نسبی ژن G6PDH و فعالیت آنزیم پراکسیداز در ژنوتیپ های حساس و متحمل برنج (Oryza sativa L.)

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

2 دانشجوی دکتری، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

3 دانشیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

4 استاد، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

5 دانش آموخته کارشناسی ارشد، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

10.22124/cr.2020.15255.1545

چکیده

تحت شرایط سمیت آهن، یکی از مهم­ترین عوامل محدودکننده تولید برنج در زمین­های کشاورزی، افزایش رادیکال­های فعال اکسیژن و در نتیجه کاهش رشد و نمو گیاه است. در این پژوهش، اثر سمیت ناشی از آهن در سطوح صفر (شاهد)، 100، 250، 400 و 500 میلی­گرم در لیتر آهن (Fe-EDTA) بر برخی صفات مرفولوژیک، بیان نسبی ژن فتوسنتزی G6PDH و فعالیت آنزیم پراکسیداز در دو ژنوتیپ Pokkali (متحمل) و IR64 (حساس) برنج در مرحله چهار­برگی تحت شرایط هیدروپونیک با محلول غذایی یوشیدا بررسی شد. آزمایش به­صورت فاکتوریل با دو فاکتور ژنوتیپ و غلظت آهن (به­ترتیب با دو و پنج سطح) در قالب طرح کاملاً تصادفی با سه تکرار انجام شد. نتایج نشان داد که برهمکنش سمیت آهن و ژنوتیپ بر تمامی صفات مطالعه شده به­جز وزن خشک ریشه، تاثیر معنی­داری داشت. تجزیه خوشه­ای با روش پیوستگی کامل (دورترین همسایه­ها)، ده تیمار مورد مطالعه را در دو گروه مجزا قرار داد. تجزیه به عامل­ها نیز نشان داد که 66 درصد از تنوع کل صفات به­وسیله دو عامل مستقل توجیه شد. اعمال تنش سبب کاهش بیان نسبی ژن G6PDH در هر دو ژنوتیپ شد، با این تفاوت که در ژنوتیپ Pokkali میزان بیان نسبی این ژن با اعمال سطوح کم تنش کاهش یافت، اما در ژنوتیپ IR64 کاهش بیان این ژن فقط در سطوح بالای تنش اتفاق افتاد. کم­ترین میزان فعالیت آنزیم پراکسیداز در هر دو ژنوتیپ Pokkali وIR64  در غلظت 100 میلی­گرم در لیتر آهن و بیش­ترین میزان فعالیت آن به­ترتیب در سطوح 400 و 250 میلی­گرم در لیتر آهن مشاهده شد. به­نظر می­رسد کاهش بیان ژن G6PDH در سطوح کم تنش و افزایش فعالیت آنزیم پراکسیداز در ژنوتیپ متحمل می­تواند میزان پراکسید­هیدروژن ناشی از سمیت آهن را کاهش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of iron toxicity on some of morphological traits, relative gene expression of G6PDH and peroxidase enzyme activity in resistant and susceptible genotypes of Rice (Oryza sativa)

نویسندگان [English]

  • Mohammad Mohsenzadeh Golfazani 1
  • Maryam Pasandideh Arjmand 2
  • Seyyed Hassan Hassani Kumleh 3
  • Habibollah Samizadeh Lahiji 4
  • Rasool Vahedi 5
  • Sahar Ramezanzadeh Bishegahi 5
1 Assist. Prof., Dept. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Ph. D. Student, Dept. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Assoc. Prof., Dept. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
4 Prof., Dept. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
5 M. Sc. Graduated, Dept. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

Under the iron toxicity conditions, one of the most important limiting factors of rice production in agricultural lands is the increase of active oxygen radicals and thus decrease of plant growth and development. In this study, the effect of iron induced toxicity at 0 (control), 100, 250, 400 and 500 mg/l (Fe-EDTA) levels were investigated on some morphological traits, relative expression of G6PDH photosynthetic gene and activity of peroxidase enzyme in two rice genotypes, Pokkali (tolerant) and IR64 (susceptible), at four-leaf growth stage under hydroponic conditions with Yoshida nutrient solution. The experiment was carried out as factorial with two factors, genotype and iron concentration (two and five levels, respectively), in a completely randomized deshgn with three replications. The results showed that the interaction of iron toxicity and genotype had significant effect on all studied traits except root dry weight. Cluster analysis using farthest neighbor divided the ten studied treatments into two separate groups. Principal component analysis showed also that 66% of the total variation was explained by two principal components. Applying iron toxicity stress reduced the relative expression of G6PDH gene in both genotypes, exept that the relative expression of this gene in the Pokkali genotype decreased with the application of low stress levels, but in the IR64 genotype, the decrease in expression of this gene observed only at high stress levels. The lowest activity of peroxidase enzyme was observed in both Pokkali and IR64 genotypes at 100 mg/l iron and the highest activity were observed at 400 and 250 mg/l, respectively. It seems that decreasing the expression of G6PDH gene at low stress levels and increasing the activity of peroxidase enzyme in the tolerant genotype can reduce the amount of hydrogen peroxide induced by iron toxicity.

کلیدواژه‌ها [English]

  • IR64
  • Pokkali
  • Real-Time-PCR
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