الگوی بیان ژن های HMA1، HMA2 و HMA9 تحت شرایط کمبود روی در ارقام گندم نان با کارایی متفاوت جذب عنصر روی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

2 دانشیار، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

چکیده

پروتئین­های مرتبط با عناصر سنگین (Heavy Metal Associated Proteins HMA:) در جذب روی (Zn) از خاک و جابه‏جایی آن در گیاهان نقش دارند. به­منظور مطالعه بیان ژن‏های HMA1، HMA2 و HMA9 در ارقام گندم نان روی- کارا و روی- ناکارا تحت شرایط کمبود روی، آزمایشی به­صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در گلخانه اجرا شد. دو رقم روی- کارا (هامون) و روی- ناکارا (هیرمند) تحت شرایط کمبود روی (صفر) و کفایت آن (5 میلی­گرم روی در کیلوگرم خاک) کشت و بیان نسبی این ژن‏ها در ریشه و برگ در دو مرحله رشدی یک ماه بعد از کشت (مرحله رویشی) و 30 درصد گلدهی (مرحله زایشی) با Real time PCR اندازه‏گیری شد. نتایج این آزمایش نشان داد که بیش­ترین میزان افزایش بیان ژن­های HMA1 (۵9/30 برابر شاهد) و ‏HMA2 (65/13 برابر شاهد) در مرحله زایشی به­ترتیب در برگ و ریشه رقم هامون مشاهده شد. در مقابل، بیش­ترین میزان افزایش بیان ژن HMA9 (83/48 برابر شاهد) در ریشه رقم هامون در مرحله رویشی مشاهده شد و در مرحله زایشی اختلاف معنی­داری بین ارقام روی-کارا و روی-ناکارا به­لحاظ میزان بیان این ژن در ریشه مشاهده نشد. بنابراین، نتایج مطالعه حاضر نشان داد که بیان ژن­های HMA1، HMA2 و HMA9 در تحمل به تنش کمبود روی خاک در ارقام گندم نان روی-کارا دخیل هستند.

کلیدواژه‌ها


عنوان مقاله [English]

Expression pattern of HMA1, HMA2 and HMA9 genes under Zn deficiency conditions in bread wheat cultivars with different Zn uptake efficiency

نویسندگان [English]

  • Kamran Moradi 1
  • Babak Abdollahi Mandoulakani 2
1 M. Sc. Student, Dept. of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Iran
2 Assoc. Prof., Dept. of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Iran
چکیده [English]

Heavy metal associated (HMA) proteins are involved in zinc (Zn) uptake from soil and its transport in plants. To study the expression pattern of HMA1, HMA2 and HMA9 genes in Zn-efficient and
Zn-inefficient bread wheat cultivars under zinc deficiency conditions, a factorial experiment in completely randomized design (CRD) with three replications was carried out in greenhouse. Two
Zn-efficient (Hamoon) and -inefficient (Hirmand) cultivars were grown under Zn deficient and sufficient (5 mg Zn per kg soil) conditions and relative expression of the studied genes in root and leaf at two growth stages, one month after cultivation (vegetative stage) and 30 % flowering (reproductive stage) were measured by real time PCR technique. The results revealed that the highest increase in the expression of HMA1 (30.9 fold more than check) and HMA2 (13.65 fold more than check) genes were observed at reproductive stage in the leaf and root of Hamoon cultivar under Zn deficiency conditions, respectively. In contrast, the highest increase in the expression of HMA9 gene (48.83 fold more than check) was found in the root of Hamoon cultivar at vegetative stage. Also, there was not a significant difference between Zn-efficient and -inefficient cultivars in term of expression of this gene in the root at reproductive stage. In conclusion, the results of the current study showed that the expression of HMA1, HMA2 and HMA9 genes are involved in soil Zn deficiency tolerance in Zn-efficient bread wheat cultivars.

کلیدواژه‌ها [English]

  • Heavy metal-associated proteins
  • Real time PCR
  • Reproductive growth stage
  • Vegetative growth stage
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