پاسخ لاین‌های موتانت برنج به تنش شوری در مرحله گیاهچهای با استفاده از صفات مورفولوژیک و نشانگرهای ریزماهواره

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

2 استاد، گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران

3 مؤسسه تحقیقات برنج کشور، رشت

4 دانش‌آموخته دکتری، گروه بیوتکنولوژی، دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران

چکیده

تنش شوری بعد از خشکی، دومین مشکل عمده و عامل محدودکننده تولید برنج است. به­منظور بررسی آثار تنش شوری بر برخی از ویژگی­های مورفولوژیک و فیزیولوژیک برنج، تعداد 41 لاین موتانت حاصل از پرتوتابی اشعه گاما به چهار رقم برنج (دو رقم محلی ایرانی هاشمی و طارم محلی و دو رقم اصلاح­شده خزر و فجر) به­همراه ارقام والدینی و دو لاین استاندارد FL478 (متحمل به شوری) و Teqqing (حساس به شوری) تحت سه شرایط شوری (شاهد، 6 و 12 دسی­زیمنس بر متر) مورد بررسی قرار گرفتند. آزمایش به­صورت فاکتوریل در قالب طرح پایه کاملاً تصادفی با شش تکرار اجرا شد. علاوه بر آن، تنوع بین ژنوتیپ­ها با دوازده نشانگر ریز‌ماهواره پیوسته با QTL بزرگ‌اثر تحمل به شوری (SalTol) بررسی شد. نتایج تجزیه واریانس، وجود تفاوت معنی­دار و قابل­توجه بین ژنوتیپ­ها را برای همه صفات مورد مطالعه نشان داد. نتایج حاصل از تجزیه خوشه­ای داده­های مورفولوژیک، ضمن تفکیک ژنوتیپ­ها به سه گروه، لاین‌های موتانت M1، M3، M7، M9، M35، M37 و M40را در کنار لاین شاهد متحمل به شوری FL478و رقم­های فجر و هاشمی و لاین‌های M47، M48، M49 و M56را در کنار لاین شاهد حساس به شوری Teqqing گروه­بندی کرد. تجزیه خوشه­ای داده­های مولکولی نیز ژنوتیپ­ها را در سه گروه اصلی قرار داد، به­طوری­که لاین­های موتانت M35، M37 و M40 به­همراه لاین شاهد FL478 به‌عنوان ژنوتیپ­های متحمل به شوری و لاین­های موتانت M36، M47، M48، M49، M54 و M56 به­همراه لاین شاهد Teqing به‌عنوان ژنوتیپ­های حساس به شوری گروه­بندی شدند. آزمون منتل میزان تشابه این دو تجزیه خوشه­ای را 65 درصد نشان داد که دلیل این مشابهت زیاد، استفاده از نشانگرهای پیوسته با QTL بزرگ­اثر تحمل به شوری SalTol بود که هر یک از آن­ها با چند صفت مطالعه شده در این تحقیق نیز پیوسته بودند. همان­طور که ملاحظه می­شود، هر سه لاین متحمل به شوری شناسایی شده در تجزیه داده­های مولکولی، در تجزیه خوشه­ای صفات مورفولوژیک نیز شناساییو هر چهار لاین حساس به شوری شناسایی شده در تجزیه صفات مورفولوژیک، در تجزیه خوشه­ای داده­های مولکولی نیز شناسایی شدند. ارزیابی نشانگرهای ریزماهواره نیز نشان داد که نشانگرهای AP3206، RM5، RM3412 و RM140آگاهی­بخش­تریننشانگرهاجهت بررسی تنوع ژنتیکی تحمل به شوری در این تحقیق بودند.

کلیدواژه‌ها


عنوان مقاله [English]

The response of rice mutant lines to salinity stress at seedling stage using morphological traits and microsatellite markers

نویسندگان [English]

  • Mirhesamoddin Hosseini 1
  • BABAK RABIEI 2
  • AliAkbar Ebadi 3
  • Mojtaba Kordrostami 4
1 Dept. of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Professor, Dept. of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Rice Research Institute of Iran (RRII)
4 Ph. D. Graduate, Dept. of Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

Salinity stress is the second major problem and limiting the rice production after drought. To investigate the effects of salinity stress on some important morphological and physiological characteristics of rice, 41 mutant lines from radiation of gama ray to four rice varieties (two Iranian local varieties, Hashemi and Tarom and two improved varieties, Khazar and Fajr) along with parental varieties and two control lines, FL478 (tolerant to salinity) and Teqing (sensitive to salinity) were studied under three salinity conditions (control, 6 and 12 dS.m-1). The experiment was carried out in factorial experiment based on completely randomized design with six replications. The variation among genotypes were also studied using twelve microsatellite (SSR) markers linked to SalTol, a major salinity tolerant QTL. The results of analysis of variance showed significant and considerable differences among the genotypes for all studied traits. The results of cluster analysis of morphological data grouped the studied genotypes into three clusters so that M1, M3, M7, M9, M35, M37 and M40 mutant lines together with the salinity tolerant line FL478 grouped into one cluster, while Hashemi and Fajr varieties and M47, M48, M49 and M56 mutant lines along with the salinity sensitive line Teqqing grouped into a similar cluster. The cluster analysis of molecular data also divided the studied genotypes into three main groups. Accordingly, the mutant lines M35, M37 and M40 together with the control variety FL478 were grouped as salinity tolerant and the mutant lines M36, M47, M48, M49, M54 and M56 together with the control variety Teqing as salinity sensitive genotypes. The Mantel test showed a high similarity of about 65%between two cluster analyses which was due to the use of tigh linked SSR markers to the SalTol, each of them were linked to the several traits studied in this research. As mentioned above, all three salinity tolerant lines identified in the molecular data analysis were also identified in cluster analysis of morphological traits and all four salinity sensitive lines identified in the morphological data analysis were also identified in molecular dataanalysis. Evaluating the microsatellite markers also showed that the AP3206, RM5, RM3412, RM140 markers were the most suitable and informative markers to assess the genetic variation of salinity tolarance in this research.

کلیدواژه‌ها [English]

  • Salinity sensitivity
  • salinity tolerance
  • SalTol
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